目的:观察内皮素-1(endothelin-1,ET-1)对人眼小梁细胞细胞骨架肌动蛋白的影响。方法:培养3代人眼小梁细胞,随机分为4组:对照组(0mol/L ET-1);ET-1低剂量组(10-9mol/L ET-1);ET-1中剂量组(10-8mol/L ET-1);ET-1高剂量组(10-7mol/L ET-1),各组细胞分别处理72h后,应用Western-blot方法检测小梁细胞骨架肌动蛋白(F-actin)的表达,并用FITC-phalloidin荧光探针观察肌动蛋白在细胞内的分布。结果:ET-1作用后人小梁细胞肌动蛋白表达明显增高,在10-9～10-7mol/L浓度范围内呈剂量依耐性,荧光探针示ET-1处理组小梁细胞肌动蛋白应力纤维和周边肌动蛋白明显增多浓集,细胞微丝附着与胞膜,细胞间连接及细胞贴壁部位连接明显增多。结论:ET-1能促进小梁细胞肌动蛋白表达,参与了小梁细胞骨架的重构。

AIM:To observe the effect of endothelin-1(ET-1) on the cytoskeleton protein F-actin of cultured human trabecular meshwork (HTM) cells.·METHODS:Cultured HTM cells were randomly divided into four groups:control group(0mol/L),low-dose ET-1(10-9mol/L) treatment group,middle-dose ET-1(10-8 mol/L) treatment group,and high-dose ET-1(10-7 mol/L) treatment group.After treated with ET-1,the expression of cytoskeleton protein F-actin in trabecular meshwork was analyzed with Western-blot and the distribution of F-actin was detected with FITC-Phalloidin probe.·RESULTS:ET-1 dose-dependently and significantly increased F-actin in trabecular meshwork cells.The F-actin stress fiber and periphery actin fiber highly increased and manifested mild reorganization after treated with ET-1;and there were much more cell-to-cell and cell-to-extracellular matrix attachments formation in ET-1 treated HTM cells than that in the untreated HTM cells.·CONCLUSION:ET-1 promoted the expression of cytoskeleton protein F-actin and induced the trabecular meshwork actin cytoskeleton reorganization.

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