目的:探讨补肾活血中药对大鼠慢性高眼压(elevated in-traocular pressure,EIOP)模型视功能损害的干预作用和机制。方法:采用烙闭上巩膜静脉法,制作大鼠EIOP模型,随机分为5组:模型对照组,补肾活血高、中、低剂量组,空白对照组。连续灌胃8wk末处死大鼠,观察其对EIOP大鼠眼压(intraocular pressure,IOP),多焦视网膜电图(multifocal electroretinogram,mfERG)和视网膜神经节细胞(retinal ganglion cell,RGC)病理形态学变化的影响。结果:造模后大鼠IOP明显升高,在造模后8wkIOP为造模前的3倍,补肾活血有轻度的降眼压的作用;对大鼠mfERG一阶函数核(first order kernel,FOK)的总波及1,2,3,4环P1波反应密度、总波P1波峰潜时、2环及3环N1波反应密度,3环、4环N1波峰潜时有恢复。结论:补肾活血中药可改善大鼠慢性EIOP模型的视功能,有助于mfERG反应密度和波峰潜时的恢复。

AIM:To observe the effect of traditional Chinese medicine(TCM) of bushenhuoxue on the rat model of chronic,moderately elevated intraocular pressure(EIOP).·METHODS:By unilaterally cauterizing episcleral vessels,the rat model of chronic,moderately EIOP was gotten.52 rats were divided into 5 groups randomly:model control group,treatment groups with high,middle,low dosage of bushenhuoxue of TCM,and normal control group.After given drugs or normal saline for 8 weeks,the rats were put to death and eyeballs were taken out.The effects were observed by intraocular pressure(IOP),multifocal electroretinogram(mfERG),and retinal ganglion cells(RGCs).·RESULTS:Elevated IOP was gotten and there was an approximately 3-fold increase in IOP for 8 weeks later comparing to pre-models building.Bushenhuoxue of TCM can lower IOP slightly;To observe the mfERG first order kernel(FOK),bushenhuoxue of TCM can improve P1 wave’s 1,2,3,4 ring’s amplitude density,P1 wave’s peak time,N1 wave’s 2,3 ring’s amplitude density and N1 wave’s 2,3 ring’s peak time.·CONCLUSION:Bushenhuoxue of TCM can protect the visual function from elevating IOP by improving mfERG’s amplitude density and peak time.So the mfERG can be a sensitivity method to evaluate its efficacy.

R285.5