目的:探讨基质金属蛋白酶-2(matrix metalloproteinase-2,MMP-2)及其抑制剂(tissue inhibitor of metalloproteinase-2,TIMP-2)在大鼠外伤性白内障形成过程中所发挥的作用。方法:正常成年SD大鼠20只,体质量250~300g,建立单眼晶状体穿通伤后外伤性白内障模型,并随机分为伤后12,24,72,168h4个时相组。用免疫组织化学染色法检测MMP-2和TIMP-2在各组晶状体上皮细胞(lens epithelial cell,LEC)中的表达情况。结果:外伤后12h晶状体开始出现混浊,并随时间延长而加重。MMP-2在各外伤组LEC中均有较强的阳性表达,而在正常对照眼中无表达。TIMP-2的表达在各组LEC中差异均无显著意义(P>0.05)。MMP-2与TIMP-2阳性表达率间无相关性(P>0.05)。结论:MMP-2参与了外伤性白内障形成的病理环节。MMP-2和TIMP-2表达失衡在外伤性白内障的发生和发展过程中发挥重要作用。

AIM: To investigate the expression and significance of matrix metalloproteinase-2(MMP-2) and tissue inhibitor of metalloproteinase-2(TIMP-2) in the rat traumatic cataract models. METHODS:Twenty healthy SD rats(weighing 250-300g) were used to establish traumatic cataract model on left eyes and then divided into post-traumatic 12, 24, 72 and 168 hours groups randomly. The expression of MMP-2 and TIMP-2 in lens epithelial cells(LEC) were examined by immunohistochemistry staining. RESULTS: In the post-traumatic 12 hours, lens opacity began to appear and aggravated as the time went by. Immunohistochemical results showed positive MMP-2 expression in the LEC of every traumatic groups. No expression of MMP-2 was detected in the compared eyes without surgery. But there was no statistical significance for TIMP-2 expression (P>0.05). There was no correlation between the expression of MMP-2 and TIMP-2 (P>0.05). CONCLUSION: MMP-2 takes part in the formation process of traumatic cataract. The imbalance between the expression of MMP-2 and TIMP-2 may play a critical role in the generation and development of traumatic cataract.

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