目的:探讨中药金钗石斛提取物中4种不同极性的生物碱(脂溶性、水溶性、低极性、弱极性)对人晶状体上皮细胞(HLEC)氧化损伤防护作用的影响。方法:将H2O2与传代培养的HLEC共同孵育复制氧化损伤模型,同时加入不同极性的金钗石斛提取物作用24h后,用四甲基偶氮唑蓝法(MTT)检测HLEC的增殖情况,流式细胞术检测其凋亡情况,并探讨不同极性及同一极性不同浓度的金钗石斛提取物对氧化损伤后细胞增殖及凋亡的影响。结果:H2O2组LEC增殖下降,金钗石斛脂溶性生物碱和水溶性生物碱高剂量组可促进氧化损伤的LEC的增殖,其中脂溶性生物碱显著增强氧化损伤的LEC的增殖(P<0.01)。脂溶性生物碱可以明显抑制H2O2诱导的LEC凋亡(P<0.01)。结论:金钗石斛脂溶性生物碱低剂量组通过抗氧化损伤而促进LEC的增殖,抑制LEC的调亡。

AIM:To investigate the protection of different polarity alkaloids (fat-soluble, water-soluble, low polar, weak polar) from dendrobium nobile on H2O2 induced damage of human lens epithelial cells (HLEC). METHODS:The H2O2 and HLEC were incubated with oxidative damage model . At the same time the different polarity alkaloid were added and sustained for 24 hours. Then detection of the proliferation of HLEC was with MTT and apoptosis was detected by FCM. RESULTS:The proliferation of LEC in H2O2 group was declined. The group of fat-soluble alkaloid and water-soluble alkaloid(25μg/L) were significantly increased compared to H2O2-induced group. The group of fat-soluble alkaloids could significantly reduce the H2O2-induced apoptosis of LEC(P<0.01). CONCLUSION: Fat-soluble alkaloids(12.5μg/L) extracted from dendrobium nobile can enhance the proliferation of LEC by anti-oxidation, and inhibit the apoptosis of LEC.

R285.5