目的:研究EGb761对视神经夹伤后兔视网膜视神经节细胞(RGC)的保护作用。方法:大白兔24只,右眼为实验组,左眼为对照组,制作视神经夹伤模型。右眼球后注射EGb761(60mg/kg),左眼球后注射等容量平衡盐液BSS。于伤后4,7,14d取材,应用病理图像分析仪计数RGC;并进行髓鞘碱性蛋白(MBP)的免疫组化染色分析。结果:伤后3~14d,两组RGC数目均下降,差异有统计学意义(P<0.01);实验组RGC数目明显高于盐水组,差异有统计学意义(P<0.01);伤后两组均有MBP阳性表达,BSS组表现为强阳性;实验组MBP表达呈下降趋势,14d表达呈弱阳性;BSS组表达也随时间有所减弱,但14d时表达仍明显;不同时间点实验组RGC阳性率均低于BSS组,差异均有统计学意义(P<0.01)。结论:EGb761能降低视神经夹伤后MBP的含量,提高RGC的存活数量。

AIM: To observe the protective effect of EGb761 on retinal ganglion cells (RGC )in rabbits with optic nerve crush injury by myelin basic protein (MBP )in rabbits’ retina. METHODS: Twenty-four rabbits were divided into experimental(right eye)and control(left eye) groups. The rabbits’ optic nerves were crushed by mini-vascular nip. EGb761 60mg/kg were injected into the retrobulbar of right eye and the same volume balanced saline solution (BSS) were injected into the retrobulbar of left eye. 4, 7 and 14 days after injury, the number of the RGC was counted by image analysis system, and the immunohisto-chemical staining of the MBP was observed. RESULTS: Three to fourteen days after injury, the number of RGC in the experimental group and control group were lower than that in normal by microscope. The differences were significant (P<0.01); the number of RGC in experimental group was higher than that in the control group. The difference was significant (P<0.01); the results of immunohisto-chemical staining: expression of MBP in both experi-mental group and control group were gradually decreased after injury. The expression of MBP in experimental group was less apparent than that in BSS group and decreased obviously at 14 days, while the expression of MBP in BSS group was still obvious at 14 days. The positive rates of RGC in different durations of experi-mental group were lower than those in the BSS group. The differences were significant (P<0.01). CONCLUSION: EGb761 can reduce the content of MBP increase, the survival rate of RGC and may have the protective effect in preventing RGC from degeneration.

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