目的:研究慢性高眼压大鼠视网膜髓鞘相关抑制蛋白No-go-A表达的变化。方法:成年雄性Wistar大鼠36只随机分为正常对照组6只和慢性高眼压组30只,应用免疫组织化学方法观察慢性高眼压大鼠视网膜不同时点Nogo-A表达的变化。结果:与正常对照组相比,慢性高眼压21d大鼠视网膜变薄,节细胞数量减少(P<0.05);Nogo-A表达增多,与形态学变化相一致(P<0.05)。结论:髓鞘相关抑制蛋白Nogo-A在慢性高眼压大鼠视网膜损伤过程中发挥了重要作用。

·AIM:To study the expressive variation of Nogo-A on rat retina in the process of chronic ocular hypertension.·METHODS:Thirty-six healthy adult male Wistars were randomly divided into control group(6 rats)and chronic hypertension group(30 rats).Chronic hypertension was created by cauterizing the superficial scleral veins.Immunohistochemistry technique was used to evaluate the expressive varieties of Nogo-A at different time points during the course of chronic ocular hypertension.·RESULTS:The success of the model was indicated by over 40% of increase in the IOP as compared with normal rats.Compared with control group,as time passed chronic hypertension group gradually had detectable morphology changes in the retina.At the 21st day of chronic ocular hypertension,retinas became thinner and the quantity of retinal ganglion cell(RGC)decreased(P<0.05).Assoicated with the morphological changes,the expression of Nogo-A was strongly increased(P<0.05).·CONCLUSION:Myelin associated protein Nogo-A plays a part in the process of chronic ocular hypertension.·

R774.1

中国辽宁省科技攻关资助项目(No.2009225021 )