目的:探讨CTGF对糖尿病大鼠视网膜内细胞凋亡的影响。方法:将60大鼠分为对照组,糖尿病4,8,12,16wk组和干预组。糖尿病大鼠应用STZ腹腔注射诱导成模。干预组大鼠于糖尿病成模后16wk玻璃体腔注射CTGFsiRNA来造成CTGF基因的沉默。取各组视网膜组织,应用RT-PCR检测视网膜内的CTGF基因表达,Tunnel法检测视网膜凋亡细胞的情况。结果:糖尿病组视网膜内CTGF表达和凋亡细胞数较正常组明显增加。凋亡发生在建模后4wk,并随着时间的延长而加重,在24wk时,凋亡细胞数为25.8个/mm2。CTGF表达于8wk时出现升高,到16wk时升高更明显。CTGFsiRNA处理后凋亡细胞数明显降低。视网膜内CTGF和细胞凋亡之间具有明显的相关性。结论:CTGF参与了糖尿病视网膜早期病变的细胞凋亡机制,CTGFsiRNA有利于改善糖尿病早期视网膜由于凋亡所致的细胞丢失。

·AIM:To detect the effect of CTGF on the apoptosis in the diabetic retina with small interfering RNAs(siRNA)targeting with CTGF.·METHODS:A total of 60 rats were divided into six groups including control group,diabetic 4,8,12,16 weeks group,and interference group.Diabetic rats were induced by STZ intra-peritoneal.At 4,8,12,16 weeks after diabetic setting up,retinas were obtained from control,diabetic rats and diabetic animals treated by intravitreal injection of CTGFsiRNA to suppress the expression of CTGF mRNA.Retinal cells apoptosis was detected by Tunnel staining and mRNA expression of CTGF was analyzed by RT-PCR.·RESULTS:The levels of CTGF and the apoptosis in the retinas of diabetic rats were significantly higher than those in the controls.Apoptosis occurred at 4 weeks after a diabetic model setting up,became serious with the diabetes developing,while CTGF elevated at 8 weeks.The cell apoptosis counts increased to 25.8cells/mm2 at 24 weeks of diabetes.SiRNA-mediated inhibition of CTGF mRNA resulted in a significant decrease in apoptosis.Significant correlations were found between CTGF and apoptosis in the retina.·CONCLUSION:These results suggest that CTGF might be involved in retinal cells apoptosis which is a characteristic of early diabetic retina.siRNA targeting CTGF seems to have the advantage of ameliorating retinal cells lost.·

R587.1

中国辽宁省教育厅立项资助项目(No.20060994)