目的:研究原花青素(procyanidins,PC)对紫外线诱导的人晶状体上皮细胞(len epithelial cells,LECs)DNA氧化损伤的保护作用。方法:采用照射剂量为15mJ/cm2的中波紫外线(UVB)分别照射用0(对照组),0.05,0.1,0.2mg/mL的PC,牛磺酸(TAU),维生素C(VC)预处理的LECs,未处理组未给予紫外线和任何抗氧化剂处理,用单细胞凝胶电泳法(SCGE)检测分析各PC组LECs DNA单链断裂的程度,并与其他各组的检测结果相比较。结果:各PC组的尾长、尾部DNA百分比、尾力矩在数值上均明显小于对照组,且与对照组相比,差异均有统计学意义(P<0.01)。各PC实验组的尾部DNA百分比、尾力矩与未处理组相比差异均无统计学意义(P>0.05)。相同浓度下,PC,TAU和VC组的各检测指标数值逐渐增大。结论:外源性PC对UVB照射诱导损伤的人LECs DNA有保护作用。PC的保护作用明显强于TAU和VC,并且在一定浓度范围内随着浓度的增加其抗氧化作用逐渐增强。

·AIM:To determine the protection of procyanidins(PC)against UVB-induced oxidative damage of lens epithelial cells(LECs)with alkaline single cell gel electrophoresis,in order to provide an experimental foundation for cataract clinical treatment.·METHODS:LECs lines of generation 4 were divided randomly into 5 groups.As untreated group:normal cultur-ed LECs;control group:LECs + UVB;three treated groups:PC treated groups:LECs + PC + UVB;taurine(TAU)treat-ed groups:LECs + TAU + UVB;VC treated groups:LECs+VC+UVB.All groups of antioxidants were divided into 3 groups according to the final concentration from low to high(0.05,0.1,0.2mg/mL).Each treated group was treated by antioxidants 2 hours before irradiated under UVB,respectively,then conventional cultivation.The LECs of treated groups and control group were all irradiated at UVB-dose 15mJ/cm2.All of LECs in every group were collected after treatment an hour respectively and analyzed with alkaline single cell gel electrophoresis.·RESULTS:The differences of tail length,percentage of tail DNA and tail moment between PC treated groups and control group were statistically significant(P<0.01).The differences of percentage of tail DNA,tail moment in each PC treated group and untreated group were not significant(P>0.05).The results of PC treated groups were signi-ficantly smaller than those of TAU treated groups and VC treated groups,The differences of tail length,percent-age of tail DNA and tail moment in various antioxidant treated groups were statistically significant(P<0.01).·CONCULSION:Exogenetic PC has protective effect to UVB-induced LECs DNA damage.The protective effect of PC is obviously stronger than those of TAU and VC,and in a certain range of concentration,the protective effect of PC has the positive relationship of dose-effect.·

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中国辽宁省自然科学基金资助项目(No.20092101);中国辽宁省教育厅重点实验室基金资助项目(No.2009S111,LS2010177)~~