目的:观察黄酮对氧化剂所诱导的视网膜色素上皮细胞(RPE)的保护作用。方法:在体研究中,预先给予5g/L黄酮滴眼液(3次/d),1wk后舌下静脉注射NaIO3诱导大鼠RPE变性,在2和4wk末,采用视网膜电图(ERG)测量C波。离体研究中,采用缺氧、H2O2、NaN3和t-BHP诱导RPE细胞损伤,并用MTT法检测细胞的存活率。结果:ERG的C波结果表明,第4wk末,黄酮抑制了由NaIO3诱导的大鼠RPE变性。离体研究结果表明,黄酮对多种氧化剂所诱导的RPE细胞损伤具有保护作用。结论:黄酮对氧化诱导的在体和离体视网膜色素上皮细胞均具有保护作用。

AIM:To investigate the effect of flavone on oxidation-induced injury in retinal pigment epithelium(RPE) cells. ·METHODS:In in vivo studies,NaIO3-induced RPE degeneration in rat eyes were treated with 5g/L flavone eye drops 3 times a day for 1 week before and 4 weeks after NaIO3 injection. At the end of 2 and 4 weeks,all rats were measured c-wave by electroretinogram(ERG). In in vitro studies,ARPE-19 cells were treated with hypoxia,H2O2,NaN3 and t-BHP to induce cell damages. MTT assay was used to measure the viable cells. ·RESULTS:The ERG c-wave results showed that flavone reversed NaIO3-induced injury at the end of 4 weeks. In vitro results showed flavone reversed the various oxidants-induced injuries in RPE cells. ·CONCLUSION:Flavone could prevent the RPE from oxidation-induced injury both in vivo and in vitro.

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