目的:探讨塞来昔布对糖尿病(diabetes mellitus,DM)大鼠凋亡抑制蛋白Bcl-2表达的影响。方法:将40只Wistar大鼠用链脲佐菌素(STZ)ip制成DM大鼠模型,随机分成DM组(n=20)和塞来昔布灌胃(celecoxib group,Cel)组(n=20)。另10只正常大鼠为正常对照组(contrast group,Con)。3mo后处死全部大鼠,制备大鼠视网膜石蜡切片,应用免疫组化法及HPIAS-1000型高清晰度彩色病理图文分析系统观测大鼠视网膜Bcl-2的表达。结果:Con组Bcl-2反应阳性弱,表达量低;DM组Bcl-2反应呈强阳性,表达增高(P<0.01);Cel组Bcl-2反应阳性减弱,表达降低(P<0.01)。结论:Cel能够减少STZ诱导的DM大鼠视网膜凋亡抑制Bcl-2蛋白表达。

AIM:To investigate the effect of celecoxib on the inhibitor of apoptosis protein Bcl-2 in retinas of early diabetic rats. ·METHODS:Forty Wistar rats were used to establish diabetic models by intraperitoneal injecting with streptozotocin,and divided into diabetes mellitus group (n=20) and celecoxib group (n=20),ten else rats were in normal control group. All of the rats were executed 3 months later. Immunohistochemistry and computer-picture analytic system were used to observe the expression of Bcl-2 protein in retinas of rats. ·RESULTS:The expression of Bcl-2 in normal group was higher than that of celecoxib group and lower than that of diabetes mellitus group(all P<0.01). ·CONCLUSION:Celecoxib can inhibit the expression of Bcl-2 in retina of diabetes mellitus.

R587.2