目的:研究豚鼠镜片诱导型近视眼视网膜、脉络膜及巩膜中RAR-β的表达变化。方法:3～4周龄花色豚鼠50只随机分成3组,空白对照组10只,离焦Ⅰ组和离焦Ⅱ组各20只。Ⅰ组和Ⅱ组豚鼠左眼作为自身对照眼,右眼戴-10.00D凹透镜,分别戴镜14,28d后除去镜片,建立近视离焦模型。镜片诱导型近视模型建立后分别处死实验组和对照组豚鼠,应用免疫组织化学、Western blot和RT-PCR技术检测RAR-β的蛋白和mRNA表达变化。结果:在豚鼠视网膜、脉络膜、巩膜内均有RAR-β存在;RAR-β蛋白及mRNA表达量在离焦组视网膜中明显增高(P<0.05),而在脉络膜及巩膜中明显下降(P<0.05)。结论:RAR-β可能参与豚鼠镜片诱导型近视眼的发生发展。

AIM:To investigate the retinoic acid receptor-β(RAR-β) expression in guinea pig eyes and study the relationship between the RAR-β expression and experimental myopia. ·METHODS:Fifty guinea pigs were randomized into the normal control group(n=10),defocusⅠgroup(n=20) and defocusⅡgroup(n=20). Guinea pigs in the defocus groups wore-10.00D lenses on the right eyes:defocusⅠgroup,wore lens for 14 days,and defocusⅡ group for 28 days. The left eyes didn’t wear lenses as self-control eyes. The refraction and axial length were measured before and after wearing lenses respectively. The protein expression of RAR-β in retina was detected by immunohistochemisty and Western blot. RT-PCR was used to observed the level of RAR-β mRNA in retina. ·RESULTS:Compared with the control eyes,the lens-worn eyes of defocusⅠand Ⅱgroups became myopic and elongated(P<0.05,respectively),and the degree of myopia was lower and axial length was shorter in groupⅠthan those of groupⅡ(P<0.05). The protein expression and the mRNA level of RAR-β in retina of the defocus groups increased significantly (P<0.05),while decreased significantly in choroid and sclera(P<0.05). ·CONCLUSION:Expression of RAR-β in guinea pig may play a role in the lens induced myopia.

R778.11