目的:检测视网膜新生血管中缺氧诱导因子-1α(HIF-1α)的表达,研究其与血管内皮生长因子(VEGF)表达的相关性。方法:以高浓度氧诱导C57BL/6J小鼠建立视网膜新生血管模型,取对照组和给氧组幼鼠眼球作荧光素血管灌注,病理切片及免疫组织化学检测,分别观察视网膜血管的改变,视网膜新生血管内皮细胞数目及HIF-1α,VEGF蛋白的表达。结果:给氧组视网膜可见大量新生血管形成,新生血管内皮细胞核数为(23.38±1.07)个,与对照组相比差异有极显著性(P<0.01)。HIF-1α蛋白表达在神经节细胞层和突破视网膜内界膜的新生血管。VEGF蛋白表达在内核层,神经节细胞层和突破视网膜内界膜的新生血管。两者表达呈显著正相关(r=0.931,P<0.01)。结论:在视网膜新生血管形成中存在HIF-1α,VEGF的高表达,且两者表达密切相关。

AIM:To investigate the expression of hypoxia-inducible factor-1α(HIF-1α) and its relationship with vascular endothelial growth factor(VEGF) in the retinal neovascularization.·METHODS:Mouse model of hyperoxia-induced ischemic retinopathy were established. A novel fluorescein-dextran perfusion method had been developed to assess the vascular pattern. The retinal neovascular response was quantitated. HIF-1α and VEGF expression in the retina was determined by immunohistochemical method. ·RESULTS:Lots of neovascularization were seen in hyperoxia group.The number of nuclei of proliferative retinal vessels increased significantly as compared with normal control group (P<0.01). The HIF-1α was expressed in the ganglion cells and retinal blood vessels;and the VEGF presented in the inner nuclear layer,ganglion cells and retinal blood vessels.·CONCLUSION:There are expression of HIF-1α and VEGF in the retinal neovascularization and they are correlated.

R774.1

中国辽宁省博士科研启动基金资助项目(No.20091115)~~