目的:观察转化生长因子β1(transforming growth factor-β1,TGF-β1)对人眼眶成纤维细胞(orbital fibroblast,OF)3H-Pro掺入的影响。方法:体外培养人OF,用0.1,1.0,5.0,10.0,100.0μg/LTGF-β1对细胞进行处理,用3H-Pro掺入法测定细胞放射性强度来判断细胞胶原合成。结果:用0.1,1.0,5.0,10.0μg/L TGF-β1作用后OF3H-Pro掺入量增加。100.0μg/L TGF-β1对OF作用相反。结论:TGF-β对人OF合成胶原有双相调节性。

AIM:To investigate the influence of transforming growth factor-β1(TGF-β1) on human orbital fibroblast(OF) by 3H-Pro absorption in vitro. ·METHODS:Human OF were cultured in vitro. Different concentrations of TGF-β1(0.1,1.0,5.0,10.0,100.0μg/L) were incubated with cultured human OF. The collagen synthesis was evaluated by 3H-Pro absorption. ·RESULTS:TGF-β1 of 0.1,1.0,5.0,10.0μg/L could promote the 3H-Pro absorption more than the control group while the concentration of TGF-β1 of 100.0μg/L had a different result.·CONCLUSION:There is a concentration-dependant effect between TGF-β1 and collagen synthesis of OF.

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