目的:探讨聚合酶链反应(PCR)技术快速诊断棘阿米巴角膜炎的价值。方法:建立棘阿米巴标准虫株的PCR检测方法,并应用于临床检测24例角膜刮片标本,结果与原虫培养及100g/L氢氧化钾湿封片镜检做比较。结果:PCR5h可检测出标本中微量棘阿米巴原虫,对照细菌、真菌、I型单纯疱疹病毒、正常人角膜均为阴性。临床标本PCR敏感性为46%,明显高于原虫培养与100g/L氢氧化钾湿封片镜检(P<0.05)。结论:PCR速度快、敏感性和特异性高,有助于棘阿米巴角膜炎的快速明确诊断。

AIM:To probe into a method of rapid diagnosis of acanthamoeba keratitis with polymerase chain reaction(PCR).METHODS:PCR was performed to detect the DNA segment of acanthamoeba from standard strain and applied to 24 scraping samples from patients’ cornea.The results were compared with protozoan culture and 100g/L KOH wet mount.RESULTS:We successfully detected acanthamoeba from standard strain and samples from patients’ cornea 5 hours after PCR,but not from bacteria,fungi,herpes simplex virusⅠ(HSV-Ⅰ)and human corneal cells.The positive rate of samples from patients was 46%,which was higher than protozoan culture or 100g/L KOH wet mount(P<0.05).CONCLUSION:PCR is fairly valuable to get rapid and definite diagnosis of acanthamoeba keratitis.

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