目的:观察人参皂苷Rg3对体外人晶状体上皮细胞SRA01/04生长的抑制作用。方法:不同质量浓度的Rg3处理体外培养SRA01/04细胞后,应用MTT比色法分析其细胞生长抑制作用;AO/EB染色法观察细胞的形态学改变;流式细胞仪测定48h细胞凋亡率。结果:Rg3在一定范围内抑制SRA01/04细胞生长,其抑制率随时间和质量浓度增加而增加。AO/EB染色可见实验组SRA01/04细胞核着黄色荧光或橘红色荧光,细胞核呈固缩状或圆珠状。流式细胞仪检测凋亡率随药物质量浓度增加而增加。结论:Rg3能有效抑制SRA01/04细胞增殖、并诱导其凋亡。

AIM:To investigate the action of Rg3 in the inhibition of human lens epithelial cells SRA01/04 in vitro and its possible mechanism.METHODS:SRA01/04 cells were cultured in medium containing 10% fetal bovine serum.10,20,40,80,120,160μg/mL Rg3 was added in medium respectively in experimental group,and the control group was set without Rg3.Effects of Rg3 on SRA01/04 proliferation were evaluated by MTT colorimetric assay.The morphology change of SRA01/04 cells was observed by the acridine orange(AO)/ethidium bromide(EB)staining.The flow cytometry was used to detect the apoptotic rates of SRA01/04 cells at 48 hours after Rg3 treated.RESULTS:The inhibiting rate of Rg3 on SRA01/04 cells was gradually enhanced in 10,20,40,80,120,160μg/mL Rg3 added group,showed a dose-dependent and time-dependent manner.We could observe the phenomena of budding and the formation of apoptosis body by fluorescent microscope.The apoptotic rates of SRA01/04 cells showed an increasing tendency in Rg3 added group with increase of Rg3 concentration(F=15.326,P=0.000).CONCLUSION:Rg3 can remarkably inhibit the proliferation and induce the apoptosis of SRA01/04 in vitro

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中国辽宁省教育厅资助项目（No.2009A457）