目的:探讨米诺环素对大鼠视神经炎视网膜神经节细胞(RGCs)的影响,并与甲基强的松龙比较。方法:选取22只雌性Wistar大鼠随机分为正常对照组和实验组,实验组又分为实验对照组(EAE组)、米诺环素组、甲基强的松龙组(MP组)。HE染色观察视神经病理改变,TUNEL法检测RGCs凋亡率。结果:EAE组视神经纤维空泡样变性,轴突不规则肿胀,大量炎性细胞浸润,轴突内空泡样变性,髓鞘松解脱落,微丝微管消失,EAE大鼠视神经病理变化符合脱髓鞘性视神经炎改变。正常大鼠几乎未见RGCs凋亡,EAE组、米诺环素组、MP组较正常组相比,差异均有显著统计学意义(P<0.01),EAE组、MP组较米诺环素组相比,差异均有统计学意义(P<0.05),MP组与EAE组间的差异有统计学意义(P<0.05)。结论:采用豚鼠脊髓匀浆诱导建立大鼠EAE模型,其视神经病理学改变符合脱髓鞘性视神经炎的表现。米诺环素可抑制脱髓鞘性视神经炎RGCs凋亡,而甲基强的松龙对脱髓鞘性视神经炎RGCs无直接保护作用。

AIM:To investigate the effect of minocycline on retinal ganglion cells(RGCs)of experimental optic neuritis in Wistar rats.METHODS:Twenty-two female experimental Wistar rats were assigned to two groups randomly:normal control group and experimental group.Then the rats of experimental group were assigned to three groups randomly:experimental control group(EAE group),minocycline group,methylprednisolone group(MP group).We observed the pathological changes of optic nerve and retina,detected the apoptosis of RGCs by TdT-mediated dUTP-biotin nick end labeling(TUNEL)method.RESULTS:In EAE group,the optic nerve fibers showed bulb-like degeneration,the axis irregularity swelling,a quantity of inflammatory cell infiltrating under light microscope,the axons showed bulb-like degeneration,myelin sheaths were loose,microtubule and microfilament disappeared under electron microscopy,the animal model we induced showed typical pathological changes of the optic nerve in EAE.Demyelination occured in the optic nerve.The area percentage of axons:there was very significant difference(P<0.01),when EAE group,minocycline group and MP group compared with normal control group.There was significant difference(P<0.05),when minocycline group and MP group compared with EAE group.There was little apoptosis of RGCs in normal rats.There was very significant difference(P<0.01),when EAE group,minocycline group and MP group compared with normal control group.There was significant difference(P<0.05),when EAE group and MP group compared with minocycline group,as well as MP group compared with EAE group.CONCLUSION:The pathological changes of optic nerve in EAE model are consistent with demyelinated optic neuritis,the EAE animal model can be used for the research of demyelinated optic neuritis.Minocycline can reduce axonal degeneration in demyelinated optic neuritis,which effect is similar with methylprednisolone.Minocycline can inhibit the apoptosis of RGCs that form the axons of optic nerve in demyelinated optic neuritis,but methylprednisolone does not have any effects on the survival of RGCs in demyelinated optic neuritis.

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