目的:探讨密蒙花总黄酮含药血浆对雄激素水平下降所致干眼症的细胞凋亡模型ARmRNA表达的影响,并应用雄激素受体阻滞剂以确定ARmRNA表达与密蒙花总黄酮含药血浆和AR受体的结合效应有关。方法:体外分离及培养泪腺上皮细胞,以H2O2诱导大鼠泪腺上皮细胞凋亡,建立雄激素水平下降所致干眼症的细胞凋亡模型。设立空白血浆组、密蒙花总黄酮含药血浆组、丙酸睾酮组,分别观察各组ARmRNA表达情况;并应用雄激素受体阻滞剂氟他胺,观察密蒙花总黄酮的拟雄激素效应。结果:密蒙花总黄酮含药血浆干预组与丙酸睾酮干预组中ARmRNA的表达增强,两组间的差异有显著性(P<0.05)。各组加入雄激素受体阻滞剂后,组间的ARmRNA表达无差异(P>0.05)。结论:密蒙花总黄酮含药血浆可促进ARmRNA表达,产生与丙酸睾酮相同的雄激素效应。ARmRNA的表达与密蒙花总黄酮含药血浆和AR受体的结合效应有关。

AIM:To establish the dry eye apoptosis model caused by the reduction of androgen levels,to explore the effect of Buddleja officinalis total flavonoids drug-containing plasma for activating the expression of AR mRNA;detect the expression of AR mRNA with the same method after using androgen receptor blockers,to determine relationship of the expression of AR mRNA with the binding effect of AR receptor in the Buddleja officinalis total flavonoids drug-containing plasma.METHODS:In vitro lacrimal gland epithelial cells were isolated and cultivated.The apoptosis of epithelial cells of rat lacrimal gland were inducted by H2O2,the dry eye apoptosis state caused by the reduction of androgen levels were established.There were the blank plasma group,Buddleia officinalis plasma total flavonoids interfere with drug-containing group,the intervention group of testosterone propionate,the expression of AR mRNA in each group was observed and androgen receptor blocker flutamide was used to explore the intended androgen effect of Buddleja officinalis total flavonoids.RESULTS:The results of Western blot showed that after the intervention of drug-containing plasma,the expression of AR mRNA in Buddleja officinalis drug-containing plasma total flavonoids intervention group and testosterone propionate intervention group were enhanced and the differences between the two groups were significant(P<0.01).After using the androgen receptor blocker in all groups,the expression of AR mRNA in each group had no difference(P>0.05).CONCLUSION:Buddleja officinalis total flavonoids drug-containing plasma can promote the expression of AR mRNA which is associated with the combination of Buddleja officinalis total flavonoids drug-containing plasma and the AR receptor,creating the same androgen effect with testosterone propionate.

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中国国家自然科学基金资助项目(No.30772824);中国教育部高等学校博士学科点专项基金资助项目(No.200805410004);中国湖南省自然科学基金资助项目(No.07JJ3049);中国湖南省科技厅科研基金资助项目(No.200-9FJ3001);2008年中国湖南省研究生创新基金资助项目