目的:观察汉防己甲素(tetrandrine,Tet)对正常结膜和翼状胬肉成纤维细胞抑制作用的异同。方法:在相同的培养条件下,传第3代的正常结膜与翼状胬肉成纤维细胞皆加入不同浓度的Tet,分别于加药后1和3d行MTT检测细胞的存活率。结果:用药后1d,4×10-5和2×10-5mol/LTet对正常结膜与翼状胬肉成纤维细胞存活率无显著性差异(P>0.05),其余各浓度的Tet对翼状胬肉成纤维细胞的抑制作用大于正常结膜成纤维细胞,正常结膜成纤维细胞的存活率大于翼状胬肉成纤维细胞(P<0.01)。用药后3d,4×10-5mol/LTet组翼状胬肉成纤维细胞的存活率大于正常结膜成纤维细胞(P<0.01);其余各浓度的Tet对两种成纤维细胞存活率无显著性差异(P>0.05)。结论:用药后3d,2×10-5mol/L及半数抑制量(10-5mol/L)以下浓度的Tet对翼状胬肉成纤维细胞起抑制作用,而对正常结膜成纤维细胞的影响较小。

·AIM:To compare the inhibitory effect of tetrandrine(Tet)on fibroblast strains respectively from normal conjunctival and pterygial specimens and provide a new thought in evaluating effect of a drug.METHODS :Different concentrations of Tet solution were added to the third passage normal human conjunctival flbroblasts(HCF)and human pterygium fibroblasts(HPF).After 1,3 days in culture,the number of cells was measured by 3-(4,5-dimethyl-2thiazol)-2,5-diphenyl-2H-tetrazolium bromid(MTT).RESULTS:At 1 day,there was no statistical difference in the number of HPF and HCF in 4×10-5mol/L and 2×10-5mol/L Tet tested groups(P>0.05).The number of HCF was larger than that of HPF in other Tet tested groups(P<0.01).At 3 day,the number of HPF was larger than that of HCF in 4×10-5mol/L Tet tested group(P<0.01).There was no statistical difference in the number of HPF and HCF in other Tet tested groups(P>0.05).CONCLUSION:In view of the increased proliferation of HPF than that of HCF,2×10-5mol/L Tet and other lower-dose Tet has damage to the normal HCF when they exhibit significant antiproliferative effect on cultured HPF at 3 day.

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中国国家自然科学基金资助项目(No.30371513);中国广东省广州市科技计划基金资助项目(No.2007J1-C0101)