目的:观察褪黑素(melatonin,MLT)对高糖诱导体外培养兔晶状体白内障形成的抑制作用。方法:选取发育正常的兔晶状体,随机分为3组:A组空白对照组、B组高糖处理组、C组实验组(在高糖处理的基础上加MLT,浓度为50μmol/L)。观察不同培养时间(48,72,96h)各组晶状体混浊程度。通过黄嘌呤氧化酶法测定超氧化物歧化酶(superoxide dismutase,SOD);化学比色法测定过氧化氢酶(catalase,CAT);硫代巴比妥酸法测定丙二醛(malondialdehyde,MDA),观察不同时间各组晶状体的生物化学变化。结果:晶状体混浊情况:培养96h后,A组晶状体无明显变化,晶状体完全透明为80%,Ⅱ级混浊为20%;B组晶状体混浊逐渐加重,晶状体Ⅳ级混浊为20%,Ⅴ级混浊为80%;C组晶状体完全透明为60%,Ⅱ级混浊为40%。晶状体生化指标的测定:C组兔晶状体组织中的SOD,CAT含量高于B组(P<0.01),C组兔晶状体组织中MDA含量低于B组(P<0.01)。结论:MLT可抑制高糖诱发的兔晶状体的氧化损伤,延缓和减轻高糖诱导的兔白内障的发生和发展。

·AIM:To observe the inhibitory effect of melatonin(MLT)on cataractous lenses of rabbits induced by high glucose.METHODS:The lenses of the cultured normal rabbits in vitro were randomly divided into 3 groups:the control group(group A),the group treated by high glucose(group B),and the group treated by high glucose and MLT in 50μmol/L(group C).The effect of the drug was observed at different time points(48 hours,72 hours,96 hours).At the same time,superoxide dismutase(SOD),catalase(CAT)and malondialdehyde(MDA)were tested.RESULTS:The observation of lenses after 96 hours culture,lenses in group A were transparent.The opacity in rabbits group C was apparently lower than that of in group B.The measurement of biochemical indicator of lenses:the levels of SOD and CAT were increased significantly in group C compared with that of group B(P<0.01).The level of MDA was significantly decreased compared with that of group B(P<0.01).CONCLUSION:MLT plays an inhibitory role in high glucose induced rabbit lens oxidation injury and can delay and ameliorate the occurrence and development of diabetic cataract.

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