目的:研究血管紧张素Ⅱ受体拮抗剂坎地沙坦对糖尿病(DM)大鼠视网膜组织VEGF和MCP-1表达的影响。方法:链脲佐菌素(STZ)制备DM大鼠动物模型36只,随机分为DM模型组和坎地沙坦治疗组,另取18只正常SD大鼠作为正常对照组,每组均随机分为4,8,12wk3个亚组。视网膜铺片联合PAS染色观察视网膜微血管形态学变化,应用SABC免疫组织化学法检测坎地沙坦对大鼠视网膜组织VEGF和MCP-1表达的影响。结果:正常对照组视网膜血管网结构清晰,走行规则;DM模型组血管迂曲阻塞,走行不规则;治疗组见血管网迂曲情况较模型组明显改善,走行较规则。在对照组和模型4wk组中大鼠视网膜组织无VEGF和MCP-1阳性表达或只呈弱阳性表达;模型8wk和12wk组两者阳性表达明显增强,且随着病程延长呈递增趋势。治疗组两者的表达则均较同时期模型组明显减弱,差异有统计学意义(P<0.05)。结论:研究血管紧张素Ⅱ受体拮抗剂坎地沙坦可降低DM大鼠视网膜VEGF和MCP-1的表达。

·AIM:To observe the effects of candesartan on the expression of vascular endothelial growth factor(VEGF)and monocyte chemotactic protein(MCP-1)in the retina of diabetic rats.METHODS:Diabetes models were established in 36 SD rats by the injection of streptozotocinum(STZ)40mg/kg with blood glucose>16.7mmol/L on the third day.Then successful model rats were randomly divided into diabetes model group,candesartan treatment group.Another 18 normal SD rats were recruited as normal control group.Every group was divided into three groups for 4,8,12 weeks.The expression of VEGF,MCP-1 protein in retina was detected by immunochemistry.RESULTS:The expression of VEGF and MCP-1 protein in retina of diabetes model group and candesartan treatment group was significantly higher than that of normal control group(P<0.05),the expression of VEGF,MCP-1 in candesartan treatment group was lower than diabetes model group(P<0.05).CONCLUSION:Candesartan injected intraperitoneally decreases the expression of VEGF and MCP-1 in retina of diabetic rats so that partly suppresses the development of diabetic retinopathy.

中国辽宁省科技攻关计划资助项目(No.2009225010-45)