目的:研究在高糖条件下,从海藻中萃取的新型多糖化合物对高糖诱导的视网膜色素上皮(RPE)细胞异常增殖的保护作用。方法:将体外培养的RPE细胞分为空白组、高糖组和多糖化合物组,空白组为正常RPE细胞培养液,高糖组为含30mmol/L葡萄糖的培养液,多糖化合物组为含30mmol/L葡萄糖和200mg/L多糖化合物的培养液。应用MTT法测量36h内不同时间点(6,12,24和36h)高糖以及多糖化合物对RPE细胞增殖影响。结果:高糖导致RPE细胞异常增殖,多糖化合物组RPE的细胞异常增殖明显得到保护,与高糖组比较差异具有统计学意义(P<0.01)。结论:从海藻中萃取的新型多糖化合物可以明显保护高糖所导致的RPE细胞的异常增殖。

·AIM:To investigate the protective effect of a novel polysaccharide compound derived from algae on high glucose-induced abnormal proliferation of retinal pigment epithelial(RPE)cells.METHODS:RPE cells were divided into 3 groups:blank control group,high glucose group and polysaccharide compound group.RPE cells were exposed with 30 mmol/L high glucose in high glucose group,and exposed with 30 mmol/L high glucose and 200mg/L polysaccharide compound in polysaccharide compound group.Cell viability of RPE cells was determined by MTT cell viability assay at different time points(6,12,24,36 hours).RESULTS:High glucose caused abnormal proliferation of RPE cells,which was abrogated in polysaccharide compound group(P<0.01).CONCLUSION:The novel polysaccharide compound derived from algae can protect RPE cells from high glucose-induced abnormal proliferation.

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