目的:观察复光颗粒对家兔受损视神经的保护作用。方法:采用视神经夹伤方法,建立家兔外伤性视神经病变(traumatic optic neuropathy,TON)模型,通过测定凋亡相关基因Bcl-2和Bax的表达,观察复光颗粒对受损视神经的影响。结果:视神经夹伤后,凋亡相关基因Bcl-2和Bax的表达及Bcl-2/Bax值在复光颗粒组间(低、高剂量组)比较无显著差异性(P>0.05)。而复光颗粒各组与模型对照组间均有显著差异(P<0.05),复光颗粒各组较模型组Bcl-2表达增加,Bax表达减少。结论:在本实验条件下,复光颗粒具有上调抗凋亡基因Bcl-2和下调促凋亡基因Bax表达的作用,可减轻视神经损伤的作用。

·AIM:To observe the protective effect of fuguang pellet on optic nerve through the research of expression of apoptosis-related genes(Bcl-2,Bax)in the rabbits after crushing the optic nerve with microforceps.·METHODS:By the method of crushing rabbit optic nerve,the models of traumatic optic neuropathy(TON)were set up.By determination of the expression of apoptosis-related genes(Bcl-2,Bax)in the rabbits’ optic nerve,the influence of the fuguang pellet on injured optic nerve was observed.·RESULTS:After the rabbits’ optic nerve was crushed,there were significant differences in the expression of apoptosis-related genes(Bcl-2,Bax)between model group and the group of the fuguang pellet(P<0.05).There were no significant differences between the group of high-dosage group and low-dosage group of the fuguang pellet.·CONCLUSION:Fuguang pellet can decrease damages of optic nerve by increasing Bcl-2 gene expression and restraining Bax gene expression in this experiment.·

R285.5