目的:评价血管内皮转化生长因子-β(transforming growth factor-beta,TGF-β)在氩激光诱导RWH大鼠脉络膜新生血管(CNV)形成过程中的表达及其意义。方法:RWH大鼠21只,随机分成3组,每组7只。单眼视网膜氩激光光凝诱导CNV形成后,分别在光凝后1,2,3wk摘除双眼球,应用免疫组织化学技术对光凝区进行因子Ⅷ相关抗原(FⅧR∶Ag)的检测以观察CNV形成,并检测CNV形成过程中TGF-β的表达,分析其变化趋势。结果:FⅧR∶Ag免疫组织化学检查结果显示,光凝后1wk开始形成CNV,3wk达到高峰。正常RWH大鼠视网膜中,TGF-β在视网膜神经节细胞层、内核层、色素上皮层、视网膜和脉络膜血管内皮细胞表达。视网膜光凝后,除上述部位外,TGF-β在外核层和脉络膜的光凝损伤区阳性表达。光凝后1～3wk,光斑区内FⅧR∶Ag和TGF-β阳性染色密度逐渐增加(P<0.05),FⅧR∶Ag与TGF-β阳性染色密度呈正相关(r=0.83,P<0.05)。结论:CNV形成与TGF-β表达正相关,TGF-β表达增多可能是CNV形成和增殖的主要原因之一。

AIM:To study the expression of vessel endothelial transforming growth factor-β(TGF-β) in choroidal neovascularization(CNV) induced by argon laser in RWH rats.METHODS:Three groups of 21 RWH rats were photocoagulated by argon laser in single eye.1 week,2 weeks,3 weeks after photocoagulation,the expression of TGF-β,and factorⅧ related antigen(FⅧR:Ag) was observed by immunohistochemical method.RESULTS:The result of FⅧR:Ag immunohistochemistry showed that CNV initially formed 1 week after photocoagulation and reached the peak till 3rd week.TGF-β expression was observed in the ganglion cells,inner nuclear layer,retinal pigment epithelial cells,the vascular endothelial cells of retina and choroid in normal rats.Besides the above part,TGF-β expressed positively in the outer nuclear layer and choroid after retinal photocoagulation.1 week to 3 weeks after photocoagulation,the positive stained density of FⅧR:Ag and TGF-β in faculous region gradually increased(P<0.05).The expression of FⅧR:Ag was positively correlated with that of TGF-β(r=0.83,P<0.05).CONCLUSION:CNV is positive correlated with the expression of TGF-β.The increased expression TGF-β may contribute to the main reason for the formation and development of CNV.

R774