目的:探讨用SD大鼠建立高氧诱导的早产儿视网膜病变(ROP)动物模型,为研究该病的发病机制及治疗提供理论基础。方法:新生SD大鼠24只分为2组,每组12只,实验组大鼠置于IPR-2小鼠隔离包,连续吸高浓度O27d后,再置于空气中饲养7d;对照组大鼠置于空气中饲养14d。通过视网膜铺片观察视网膜血管、组织切片HE染色观察视网膜新生血管、免疫组织化学观察VEGF和CD34蛋白的表达。结果:视网膜铺片显示实验组见大量无灌注区及新生血管芽,较对照组明显多。HE染色显示实验组内皮细胞数较对照组明显多。免疫组织化学表明CD34和VEGF在突破内界膜的细胞呈阳性表达。VEGF半定量测定显示实验组平均灰度值较对照组低,面密度值较对照组高。两组鼠生长发育均未见明显异常。结论:高氧诱导SD大鼠成功产生视网膜新生血管,可作为探究ROP疾病发生机制和治疗方法的可靠动物模型。

AIM:To investigate the SD rats which were used to establish hyperoxia-induced retinopathy of prematurity(ROP) model,providing experimental basis to study the pathogenesis and treatment of disease.METHODS:Twenty-four newborn SD rats were divided into two groups,one group were placed in IPR-2 mice separate package where given hyperoxia [(75±2)% O2] from postnatal day 1 to 7(P1-7) and after then,exposed to room air(P8-14),the other group placed in room air(P1-14).At P7,P14,retinal flatmounts were obtained to study the retinal vascular pattern;at P14,the eye-tissue-sections were obtained and stained with hematoxylin-eosin to analyze the extraretinal neovascularization;stained immunohistochemically to evaluate the retinal expression of the vascular endothelial growth factor(VEGF),CD34 protein under light microscopy.RESULTS:Retinal flatmounts:A large number of the capillary-free area and neovascularization were observed in experimental group,which was more than that in control group.Tissue-sections stained with hematoxylin-eosin:The number of vascular cell nuclei in experimental group was obviously more than that in control group.Immunohistochemical study:CD34 and VEGF protein expressed positive in the extraretinal vascular cells anterior to the internal limiting membrane.VEGF semi-determination showed the mean gray scale values was lower in experimental group than that in control group,but the area density values in experimental group was higher than that in control group.The development condition of rats was normal.CONCLUSION:The SD rats with the hyperoxia-induced animal model of ROP can successfully produce retinal neovascularization,and the model can be a reliable animal model as exploring mechanisms and treatment of disease.

R774.1

中国山东省科技攻关计划资助项目(No.2008GG-30002031)~~