目的:探讨紫药水在真菌性角膜炎诊断中的应用。方法:我院2009-01/2010-12诊断为真菌性角膜炎病例70例,行角膜刮片,标本分别行紫药水染色、KOH湿片检查和真菌培养及菌种鉴定。以培养结果做为确诊真菌性角膜炎的"金标准"。结果:真菌性角膜炎病例70例中58例检出真菌,阳性率82.9%。紫药水染色法查找真菌阳性率61.4%,灵敏度67.2%,特异度29.6%,假阳性率33.3%,假阴性率32.8%,正确指数-3.1%,阳性预测值90.7%,阴性预测值29.6%。KOH湿片法查找真菌阳性率44.3%,灵敏度44.8%,特异度17.9%,假阳性率41.7%,假阴性率55.2%,正确指数-37.2%,阳性预测值83.9%,阴性预测值17.9%。结论:紫药水染色法是真菌性角膜炎早期诊断的快速、有效方法。

AIM:To investigate the application of Methylrosanilinium Chloride in the diagnosis of fungal keratitis.METHODS:Seventy fungal keratitis cases were collected from January 2009 to December 2010.The corneal scraping specimens were performed Methylrosanilinium Chloride staining and 100g/L KOH wet mount before microscopic observation,fungal culture and strain identification.The cultivation results were regarded as the "gold standard" of fungal keratitis diagnosis.RESULTS:The cultivation results of 58 cases were positive among 70 samples of fungal keratitis,the positive rate was 82.9%.Methylrosanilinium Chloride staining showed the positive rate was 61.4%,sensitivity 67.2%,specificity 29.6%,false positive rate 33.3%,false negative rate 32.8%,the correct index-3.1%,positive predictive value 90.7%,negative predictive value 29.6%.10% KOH wat mount showed the positive rate was 44.3%,sensitivity 44.8%,specificity 17.9%,false positive rate 41.7%,false negative rate 55.2%,the correct index-37.2%,positive predictive value 83.9%,negative predictive value 17.9%.CONCLUSION:Methylrosanilinium Chloride staining is a fast,effective way for the early diagnosis of fungal keratitis.

R772.21

中国福建省自然科学基金资助项目(No.2009-J05063);中国福建省科技平台建设基金资助项目(No.2010-Y2003)~~