目的:探讨ZnSO4对紫外线诱导的大鼠晶状体氧化损伤的保护作用。方法:离体培养大鼠晶状体,随机分为三组:(1)阴性对照组(正常组);(2)阳性对照组(紫外线照射);(3)实验组(紫外线照射+ZnSO4)。观察晶状体的混浊程度,晶状体组织中GSH-Px和SOD的含量,晶状体上皮细胞中HSP70表达的变化。结果:实验组晶状体混浊程度较同时段阳性对照组轻,差异有统计学意义(P<0.05)。紫外线照射后,晶状体上皮细胞中HSP70的表达明显增加,实验组晶状体上皮细胞中HSP70的表达较同时段的阳性对照组显著增加,差异有统计学意义(P<0.05)。阳性对照组中GSH-Px和SOD含量显著低于同时段阴性对照组,差异有统计学意义(P<0.05);实验组中GSH-Px和SOD含量明显高于同时段阳性对照组,差异有统计学意义(P<0.05)。结论:在晶状体中,ZnSO4可诱导HSP70的表达进而对晶状体的氧化损伤起到保护作用。

AIM:To investigate the protective effect of ZnSO4 on ultraviolet-induced oxidative damage of rat lens.METHODS:Rat lens were randomly divided into 3 groups:negative control group (normal group),positive control group (UV radiation group) and experimental group (treated by UV radiation and ZnSO4).After cultured,the turbid level of lens was observed;the contents of glutathione peroxidase(GSH-Px) and superoxide dismutase(SOD) in tissue as well as the expression changes of heat shock protein 70(HSP70) in rat lens epithelial cells were determined.RESULTS:The turbid level of lens in experimental group was lower than that in positive control group at the same time,the differences were statistically significant (P<0.05).The expression of HSP70 in experimental group significantly increased after UV radiation,there was significant difference compared with that in positive control group (P<0.05).The contents of GSH-Px and SOD in positive control group were much lower than that in negative control group,the differences were statistically significant (P<0.05),while the contents of GSH-Px and SOD in experimental group were significantly higher than that in positive control group,the differences were statistically significant (P<0.05).CONCLUSION:ZnSO4 can induce HSP70 expression in rat lens epithelial cells,which may play a vital role in lens oxidative damage protection.

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