目的:采用基因表达谱芯片技术研究形觉剥夺近视猴视皮质中基因表达的变化。方法:将8只20～40天龄的健康幼恒河猴分为形觉剥夺组和对照组。形觉剥夺组4只猴右眼配戴散射镜片,另1眼配戴0D镜片;对照组4只猴双眼均配戴0D镜片。戴镜12wk后,形觉剥夺组和对照组猴取视皮质,用基因表达谱芯片技术对形觉剥夺猴视皮质基因表达的变化进行分析。结果:与对照组相比,形觉剥夺组中表达下调的基因明显多于表达上调的基因,其中包括Egr-1,NMDA和GABA等基因。结论:形觉剥夺性近视猴视皮质中存在基因表达被抑制的趋势。

AIM:To investigate the gene expression changes in the visual cortex of form-deprivation myopic monkeys using gene expression chip microarray techniques.METHODS:Eight healthy infant rhesus monkeys,between 20 and 40 days of age,were divided into form-deprivaton group(n=4) and control group(n=4).Monkeys from form-deprivation group wore diffuser spectacle lens over their right eyes,and 0D lens over their left eyes.Monkeys from control group wore 0D lens over both of their eyes.After 12 weeks,gene expression changes in the visual cortex of form-deprivation and control monkeys were analyzed using gene expression chip microarray techniques.RESULTS:In comparison with control group,genes that were down-regulated were significantly more than those that were up-regulated in form-deprivation group,including Egr-1,NMDA and GABA genes which had been proved to be myopia related genes.CONCLUSION:Gene expression is significantly inhibited in the visual cortex of form-deprivation myopic monkeys.And gene expression chip microarray technique is a useful tool to screen the possible myopia-related candidate genes.

R778.11

中国广东省医学科研基金资助项目(No.B2008053)~~