目的：为了筛选适合结膜松弛症结膜成纤维细胞培养的方法，对不同培养基培育及杞精明目汤药物血清作用前后的结膜松弛症和正常球结膜成纤维细胞进行观察。 方法：收集结膜松弛症松弛结膜组织16例、翼状胬肉组织11例和正常球结膜组织5例，观察成纤维细胞的生长状态，筛选最适合结膜松弛症成纤维细胞生长的培养基。 结果：采用DMEM/F12培养基可使结膜松弛症成纤维细胞少量从组织块内溢出，但不能传代。采用含100mL/L胎牛血清、1μL/mL成纤维细胞生长添加物（FGS）的DMEM-H培养基可使结膜松弛症成纤维细胞顺利生长，且能够传代。 结论：最适宜培养结膜松弛症成纤维细胞的培养基是含100mL/L胎牛血清、1μL/mL FGS、100U/mL青霉素、100μg/mL链霉素的DMEM-H培养基。

To observe the fibroblasts of normal bulbar conjunctival and conjunctivochalasis cultivated by different medium as well as qijingmingmu soup serum in order to screening the appreciate method for the cultivation of fibroblasts of conjunctivochalasis. METHODS: We collected loose conjuctiva tissue from 16 cases of conjunctivochalasis patients, pterygium tissue from 11 cases, and normal conjuctiva tissue from 5 cases. The growth state of fibroblast was observed to select the most suitable medium for the growth of fibroblasts of conjunctivochalasis. RESULTS: DMEM/F12 can make a few fibroblast of conjunctivochalasis overflow from the tissue, but can not subculture. DMEM-H(contaied 100mL/L FBS, 1μL/mL FGS) can make the fibroblast of conjunctivochalasis grow smoothly, and can transfer. CONCLUSION: The most appropriate training medium for the fibroblast of conjunctivochalasis is DMEM-H, which adds 100mL/L fetal bovine serum, 1μL/mL FGS, 100U/mL penicillin, and 100μg/mL streptomycin.

中国上海市普陀区卫生系统自主创新科研基金资助项目（No.PTKW2011-B-112）