目的: 探讨外源性给予二硫苏糖醇（DTT）对于紫外线诱导SD大鼠白内障的保护作用。 方法:32只6周龄SD大鼠（约180g ）分为4组：第一组为模型组，每天辐射300nm紫外线B（ultraviolet B）15min，辐射强度1×10³μW/cm²，连续辐射7d；第二组为实验组，辐射UVB联合每天腹腔注射DTT溶液1mL（腹腔注射于辐射30min前）；第三组实验组同第二组外，7d辐射结束后继续用DTT 1wk；第四组为空白对照组，不辐射UV-B，每天腹腔注射等量的生理盐水。 结果:腹腔注射DTT可以明显减少紫外线辐射引起的脂质过氧化，提高过氧化物酶（SOD）、还原型谷胱甘肽（GSH）含量，并且可以降低晶状体中钙离子（Ca²⁺）和乳酸脱氢酶（LDH）含量。 结论:二硫苏糖醇对紫外线诱导白内障SD大鼠具有良好的抑制作用.

AIM: To study the effect of exogenously administered dithiothreitol (DTT) on eye lens of adult rats exposed to ultraviolet radiation B(UV-B) from 300nm irradiation. METHODS: Thirty-two male SD rats aged 6 weeks (body weight about 180g) were randomly divided into four groups of 8 rats each. The first group, the model group, was exposed daily to ultraviolet radiation from 300nm at 1×10³μW/cm²for 15 minutes for one week. The second group, the experimental group, exposed in the same way as the first group, received 400μg DTT in 1mL saline 30 minutes prior to radiation, daily. The third group was also exposed to UV-B, and treated only with 400 μg DTT in 1mL saline for another week. The fourth group was kept as the control group and received a daily vehicle injection.  RESULTS: DTT injection with radiation significantly reduced lipid peroxidation, Ca²⁺ and LDH. When DTT was injected after radiation, SOD and GSH-px enzyme activities increased significantly. Also DTT significantly reduced the turbidity of the cortical cataract. CONCLUSION: Our data shows that DTT has obvious inhibitory action on ultraviolet cataract.

军队基金(No.2009ZXJ09004)