目的：探讨MK-801对大鼠慢性高眼压视网膜神经节细胞(retinal ganglial cells, RGC)的保护作用。 方法：制备SD大鼠慢性高眼压模型，36只SD大鼠随机分为空白对照组、慢性高眼压＋生理盐水对照组、慢性高眼压＋MK-801处理组，各组12 只。观察并比较各组大鼠在不同时间点（处理后1, 4, 7和10d）谷氨酸含量、视网膜总厚度和内层厚度、RGC数目及RGC凋亡情况。 结果：在各时间点，慢性高眼压大鼠（MK-801处理组和生理盐水对照组）视网膜谷氨酸含量均较空白对照组高（P<0.05）； MK-801处理组视网膜谷氨酸含量比同期生理盐水对照组明显降低，差异有统计学意义（P<0.05）。HE染色表明MK-801处理组和空白对照组视网膜总厚度和内层厚度均大于生理盐水对照组（P＜0.05），RGC数目高于生理盐水对照组（P＜0.05）。MK-801处理组RGC 层凋亡阳性细胞的表达少于生理盐水对照组。 结论：MK-801可通过阻断谷氨酸介导的神经毒性作用，对慢性高眼压RGC起到保护作用。

AIM:To investigate the the protective effect of MK-801 on retinal ganglial cells（ RGC ) of SD rats with chronic ocular hypertension. METHODS:To prepare chronic ocular hypertension model of SD rats，36 SD rats were randomly divided into 3 groups： blank control group，chronic elevated intraocular pressure + saline control group，chronic elevated intraocular pressure + MK-801 treatment group (n=12). The glutamate acid content, retinal thickness and the inner layer thickness，number of retinal ganglion cell,retinal ganglion cell apoptosis of each group were observed and compared at different time points ( 1day, 4, 7and 10days after treatment) . RESULTS:Retinal glutamate acid levels increased (P<005) in SD rats with chronic elevated intraocular pressure and those in MK-801 treatment group were significantly lower than the same period in normal saline control group (P<0.05). HE staining showed that retinal thickness and the inner layer thickness of MK-801 treatment group and blank control group were greater than the saline control group (P<0.05), the numbers of RGC were higher than the saline control group (P<0.05). The positive expression of apoptotic RGC in MK-801 treated group were less than the saline control group.  CONCLUSION:MK-801 could protect the RGC of chronic elevated intraocular pressure by blocking the glutamate-mediated neurotoxicity.

中国十堰市科技局2008年资助项目