目的:初步探讨环孢霉素A（CsA）对葡萄膜炎中IL-17⁺细胞的作用机制。 方法：抽取HLA-B27阳性葡萄膜炎患者外周血，同时建立正常人为对照组，分离外周血单核细胞，体外培养，并加入不同浓度CsA，通过ELISA检测不同浓度CsA对IL-17分泌的影响，通过细胞内细胞因子染色的方法检测CsA对IL-17⁺细胞活化的影响。 结果：CsA可以有效抑制IL-17的分泌，且随着浓度增高抑制程度增加；但是CsA不影响IL-17⁺细胞对CD69的表达。 结论：CsA可以通过抑制IL-17的分泌来实现对HLA-B27阳性葡萄膜炎的治疗。

AIM： To preliminarily discuss the mechanisms of cycloslporinc(CsA) on the IL-17⁺ cells in uveitis. METHODS: Fifteen HLA-B27 uveitis patients with active uveitis were involved in this study. Blood samples were taken from these patients for analysis of IL-17. The levels of IL-17 in the supernatants of PBMCs from patients before treatment cultured without or with CsA at different concentrations were detected by enzyme-linked immunosorbent assay (ELISA). Intracellular staining with flow cytometry was used to evaluate the expression of CD69 on the IL-17-producing cells in different groups. RESULTS: The results showed that CsA inhibited the secretion of IL-17,but it did not influence the expression of CD69. CONCLUSION: Our findings showed that one of the mechanisms of CsA on the treatment of HLA-B27 uveitis is the inhibition of the secretion of IL-17.

广东省卫生厅资助项目(No.B2010294); 深圳南山区科技局资助项目（No.南科卫2009015）;深圳市科技计划资助项目（No.201102127）