目的:探讨眼附属器B细胞非霍杰金淋巴瘤(B-cell non-Hodgkin lymphoma, NHL)中Skp2,p27和PTEN的表达。方法:收集1995年到2011年青岛大学附属医院眼科石蜡包埋标本,用免疫组化法分别检测眼附属器B细胞NHL(n=30) 标本中Skp2,p27和PTEN的表达,以眼部反应性淋巴组织增生(n=10)作为对照组。以患者的年龄、性别、发病部位,病理类型作为眼附属器B细胞NHL的的分类标准。结果:Skp2,p27和PTEN的表达与患者的年龄、性别、发病部位无关,而与病例类型有关。眼附属器B细胞NHL Skp2表达率与眼部反应性淋巴组织增生相比显著增高。 p27,PTEN表达率与反应性淋巴组织增生相比显著降低。随眼附属器B细胞NHL病理分级的提高,Skp2的表达显著增高,p27和PTEN的表达显著降低。在黏膜相关淋巴组织结(mucosa-associated lymphoid tissue, MALT)外边缘区B细胞淋巴瘤（diffuse large B-cell lymphoma, DLBCL）中,Skp2分别与p27,PTEN成负相关,p27和PTEN成正相关。结论: Skp2的表达升高,p27,PTEN蛋白的缺失以及可能与眼附属器B细胞NHL的发生有关；其中在MALT外边缘区DLBCL中,三种蛋白存在相关性。联合三种蛋白的检测眼附属器B细胞NHL的不同病理类型有重要意义。

AIM: To investigate the expression of Skp2,p27 and PTEN proteins in B-cell non—Hodgkin lymphoma(NHL) of ocular adnexa.METHODS: Paraffin sections were collected from patients suffering from B-cell NHL (n=30) and lymphadenosis (n=10) of ocular adnexa in Qingdao Affiliated Hospital from 1995 to 2011. Features (age, gender, pathogenetic locations, pathological type) can be used as factors to classify 30 cases of B-cell NHL. Lymphadenosis sections were selected to the control group. The expression of Skp2,p27 and PTEN proteins were detected by immunohistochemistry and the positive expression rate between lymphomas and lymphadenosis was compared. Spearman rank correlation was used to estimate the relationships among Skp2, p27 and PTEN in ocular lymphoma. RESULTS: The expression of three proteins were not related to age, gender, pathogenetic locations but related to pathologic type of tumors. In B-cell NHL specimens, the expression of p27^kip1 and PTEN was lower than that in lymphadenosis (P<0.05), while the expression of Skp2 was higher.Skp2 labeling frequency was increased dramatically in plasmacytoma(PL) and diffuse large B-cell lymphoma(DLBCL) compared with mucosa-associated lymphoid tissue(MALT)(P<0.01). While p27^kip1 and PTEN labeling frequency in PL and DLBCL was significantly lower than that of MALT(P<0.05,P<0.01). There was a negative correlation between p27^kip1 and Skp2 in MALT(r=-0.134, x²=12.58,P＜0.05）while there was a positive correlation between p27^kip1 and PTEN（ r=0.828, x²=20.66,P＜0.05）．The relationship between Skp2 and PTEN was also negative(r=-0.883, x²=20.52,P＜0.05).CONCLUSION: Over expression of Skp2 and lower expression of p27^kip1 and PTEN play an important role in the tumorigenesis and different pathologic type in ocular adnexal tumors. The expression of three proteins correlates with each other in MALT. Combined examination of Skp2,p27 and PTEN is a valuable marker to predict the presence of the B-cell NHL of ocular adnexa and differentiate the pathologic type of tumors.