方法:新生SD大鼠视网膜进行体外Müller细胞的培养并建立高压气体冲击损伤模型,检测细胞致伤后早期胶质纤维酸性蛋白(GFAP)表达变化,以及乳酸脱氢酶(LDH)的释放量和细胞凋亡率的变化。

结果:致伤后3h左右GFAP的表达量明显增强,以后24h内持续性高表达,并与损伤强度呈正比(P<0.05); LDH的释放增加主要出现在损伤后30min内; 损伤后30min各组致伤细胞凋亡率呈非显著性差异(P>0.05),3h后细胞凋亡率增高,6h后细胞凋亡率最高。

结论:Müller细胞形态的改变和细胞膜通透性的改变可能是触发GFAP 表达增强的因素之一。

METHODS: A model of high air pressure wave traumatic injury in cultured retinal Müller cell was established, the expression of glial fibrillary acidic protein(GFAP)after cell injury was tested by immunochemistry. The change of lactate dehydrogenase(LDH)activity and cell apoptosis rate after cell injury were observed. RESULTS: Increased expression of GFAP changes was observed 3 hours after injury. Then it maintained high level of expression within 24 hours, the results were related with injury degree(P<0.05). LDH release increased within 30 minutes after injury. The apoptosis rate had non-significant difference within 30 minutes after injury(P>0.05). The percentage of apoptotic cells increased 3 hours after injury and reached the highest 6 hours after injury.

CONCLUSION: The changes of Müller cell shape and the permeability of cell membrane maybe one of factors to trigger the increasing expression of GFAP.