目的:研究细胞周期蛋白激酶抑制因子p21和p27蛋白在不同年龄段兔晶状体上皮细胞（lens epithelial cells,LECs）中的表达规律。方法:分别取10,20,30周龄兔晶状体前囊膜组织,采用RT-PCR和Western-blot检测该组织中p21和p27的mRNA水平和蛋白水平。结果:兔晶状体前囊膜组织中p21与p27的mRNA水平和蛋白水平相似,青年最高,老年次之,中年最低。p21和p27在青年、中年及老年兔LECs中的表达水平有差异（P<0.05）。结论:p21和p27在兔LECs周期调控过程中可能起着平衡作用,对防止PCO的研究具有一定参考价值。

AIM:To investigate the expression of cyclin dependent kinase inhibitors p21 and p27 in different ages of rabbit lens epithelial cells(LEC). METHODS:p21 and p27 in lens anterior capsule membranes in 10, 20 and 30 weeks old rabbits were measured by RT-PCR and Western blot in levels of mRNA and protein. RESULTS:p21 in rabbit lens anterior capsule membranes had similar expression with p27 in levels of mRNA and protein. Their expressions were highest in youth rabbits, while lowest in middle aged rabbits. The differences of both p21 and p27 among different ages of rabbit LEC were significant(P<0.05). CONCLUSION:p21 and p27 may play a balancing role in the process of cycle regulation in rabbit LEC, which provides a reference for researches related to prevent posterior capsular opacification(PCO).

国家自然科学基金项目（No.81070715）;福建医科大学苗圃科研基金（No.2010MP014）