目的：通过建立实验性有色兔脉络膜新生血管(CNV)的动物模型，研究蛴螬提取物对血管生成素1(Ang1)和色素上皮衍生因子(PEDF)表达的影响，从而探讨其对CNV的抑制作用。

方法：选取40只有色兔，随机分成5组，A组：健康空白组； B组：模型组； C组：维生素E组； D组：驻景丸加减方组； E组：蛴螬提取物组。每组8只16眼，通过氩激光光凝方式建立CNV模型。激光光凝后24h； 7，14，21，28d行眼底彩色照相； 7，14，21，28d行荧光素眼底血管造影(FFA)； 14，28d行光学相干断层扫描(OCT)。然后将每组家兔随机分成2批，分别于14，28d用空气栓塞法处死并摘取眼球后段组织行切片、HE染色，光镜下观察视网膜组织病理形态学改变，并行Ang1和PEDF免疫组织化学染色，以研究蛴螬提取物对CNV的抑制作用。

结果：Ang1含量表达测定显示，空白组较实验组低(P<0.05)，实验组中驻景丸加减方组和蛴螬提取物组较其它三组有显著性差异(P<0.05)，蛴螬提取物组较驻景丸加减方组低，但无显著性差异，蛴螬提取物组28d较14d有显著性差异(P<0.05)。PEDF含量表达测定显示，空白组较实验组高(P<0.05)，实验组中驻景丸加减方组和蛴螬提取物组较其它三组有显著性差异(P<0.05)，蛴螬提取物组较驻景丸加减方组高，但无显著性差异，蛴螬提取物组28d较14d有显著性差异(P<0.05)。

结论：蛴螬提取物对实验性有色兔CNV中Ang1的高表达存在良性影响，能有效干预PEDF的降低，保护视网膜组织，对CNV具有抑制作用。

AIM:To study the influence of holotrichia extractive to angiogenin1(Ang1)and pigment epithelium derivation factor(PEDF)expression and probe its inhibiting effect to the choroidal neovascularization(CNV)though establishing CNV model of experimental colored rabbits.

METHODS: Forty colored rabbits were selected and divided into 5 groups randomly, including blank group, model group, vitamin E group, zhujingwanjiajianfang group and the holotrichia extractive group. There were 8 rabbits in each group(16 eyes). CNV model was established through the method of argon laser photo-coagulation. Then optic fundus colored pictures were taken after 24 hours, 7, 14, 21 and 28 days, fluorescence fundus angiography(FFA)was performed after 7, 14, 21 and 28 days and optical coherence tomography(OCT)was performed after 14 and 28 days. Then the rabbits were divided into 2 parts each group randomly, and executed by aeroembolism after 14 and 28 days. Their eyes' posterior segment tissues were selected to carry on organized microtome section, hematoxylin and eosin staining and Ang1, PEDF immunohistochemistry staining to analyze depressant effect of holotrichia extractive to CNV.

RESULTS: Determination of Ang1 content expression showed, blank group's content was the least in all groups(P<0.05), the content of holotrichia extractive group and zhujingwanjiajianfang group were less than others in experimental groups(P<0.05), holotrichia extractive group's content was less than zhujingwanjiajianfang group's content, but there was no significant difference, holotrichia extractive group's content between the 14 days and the 28 days has significant difference(P<0.05). Determination of PEDF content expression showed, blank group's content was the highest in all groups(P<0.05), the content of holotrichia extractive group and zhujingwanjiajianfang group were higher than others in experimental groups(P<0.05), holotrichia extractive group's content was higher than zhujingwanjiajianfang group's content, but there was no significant difference, holotrichia extractive group's content between the 14 days and the 28 days had significant difference(P<0.05).

CONCLUSION: The holotrichia extractive has active influence for the high experiment of Ang1 in CNV of the experimental colored rabbits, it can intervene reduction of PEDF and protects retinal tissue, and thus it has depressant effect to CNV.

国家中医药管理局重点学科建设项目; 湖南省教育厅科研基金资助项目(No.09C729); 湖南省中医药科研基金资助项目(No.2010012); 湖南省教育厅重点学科建设项目