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[摘要]
目的：检测视觉发育敏感期单眼形觉剥夺大鼠视觉电生理功能，并观察其视皮质超微结构。
方法：健康14日龄SD大鼠20只，随机分为两组，实验组及对照组各10只。实验组大鼠缝合封闭单侧眼，制作单眼形觉剥夺动物模型。与对照组在同等自然光照环境下饲养至45日龄。分别对两组鼠进行电生理检测，并使用透射电镜观察两组大鼠视皮质的超微结构。
结果：正常大鼠F-VEP呈现典型的NPN波形，P1波峰潜时短，波峰陡直。形觉剥夺大鼠F-VEP检测结果：P1波峰潜时明显延长，波峰显著降低。电镜观察单眼形觉剥夺动物视皮质神经元的超微结构发现：形觉剥夺动物视皮质中神经元的超微结构受到破坏。
结论：单眼形觉剥夺可以影响大鼠视觉电生理的变化，使P波峰的潜时延长，振幅降低，这一改变是形觉剥夺性弱视视觉生理功能改变的体现； 单眼形觉剥夺可以引起大鼠视皮质神经元超微结构破坏，这一改变是形觉剥夺性弱视的形态学基础。
[Key word]
[Abstract]
AIM: To detect the visual electrophysiological function and observe the ultrastructure in the visual cortex of sensitive period monocular deprivation rats.
METHODS: Totally 20 SD rats of 14-day-old were randomly divided into two groups, ten for each group. Rats of experimental group were sutured unilateral eye as monocular deprivation animal models and reared in the same natural light environment with the control group for 45 days. Both two groups received electrophysiological testing, visual cortex ultrastructure was observed by the transmission electron microscope.
RESULTS: Electrophysiologic study on ocular deprived animal model, F-VEP showed typical NPN wave form in normal rats, P1 wave peak latency was short, wave crest was steep and straight. Detection in ocular deprived rats showed that P1 wave peak latency lengthened obviously, wave crest depressed significantly. Observation of ultrastructure of visual cortical neurons in monocular deprived animals by electron microscope showed that it was destroyed seriously.
CONCLUSION: Monocular deprivation can affect rats' visual electrophysiological function. It makes F-VEP P1 wave peak latency of model rats obviously lengthen, amplitude of vibration significantly degrades. Monocular deprivation can destroy rats' visual cortex neurons ultrastructure and this change is the morphological basis of monocular deprivation amblyopia.
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