方法：采用烙闭上巩膜静脉法，烙闭大鼠3支上巩膜静脉，制作大鼠慢性EIOP模型，随机分为3组：空白组，模型组，给药组。连续灌胃8wk，并于8wk末处死大鼠，观察其对EIOP大鼠眼压(intraocular pressure，IOP)，初级视皮质(primary visual cortex，PVC)尼氏小体含量、神经元细胞超微结构影响。

结果： 本实验采用的烙闭上巩膜静脉的造模方法使大鼠眼压明显升高(P<0.01)，与造模前比较差异有显著统计学意义(P<0.01)； PVC病理切片半定量分析表明：模型组尼氏小体总面积34941±8482.1·S/μm²、平均光密度152.8±27.97、积分光密度11993±3084.8，与空白组(总面积55742±6348.1·S/μm²、平均光密度304.04±100.1、 积分光密度18219±3548.9)比较均有显著统计学意义(均为P<0.05)，模型组Nissl小体总面积、平均光密度、积分光密度与给药组(总面积46406±5989.3·S/μm²，平均光密度251.05±77.41，积分光密度16899±4040.6)比较，差异均有统计学意义(均P<0.05)。

结论：补肾活血中药通过增强尼氏小体的表达、改善神经元细胞超微结构而促进EIOP大鼠初级视皮质损伤的修复。

METHODS: The rat model of chronic EIOP was established by unilaterally cauterizing 3 episcleral vessels, then 30 rats were divided into 3 groups randomly: control group, model group, and treatment group. After given drugs or normal saline for 8 weeks, the rats were put to death. The effect of intraocular pressure(IOP), content of Nissl bodies and ultrastructure of neuron cell in the PVC was observed.

RESULTS: Unilaterally cauterizing episcleral vessels increased IOP of the rat modle obviously, there was significant difference compared with preoperative(P<0.01). Semi-quantitative pathological analysis showed that Nissl body of total area in the model group was(34941±8482.1)S/μm²,mean optical density was(152.8±27.97), integrated optical density was(11993±3084.8), compared with the control group, total area was(55742±6348.1)S/μm²,mean optical density was(304.04±100.1),integrated optical density was(18219±3548.9), there were statistically differences(all P<0.05). There were statistically difference in Nissl body of total area, mean optical density and integrated optical density between model group and treatment group, total area was(46406±5989.3)S/μm²,mean optical density was(251.05±77.41),integrated optical density was(16899±4040.6, all P<0.05).

CONCLUSION: TCM of Bushenhuoxue can repair PVC's damage in the rat model of chronic EIOP by enhancing expression of Nissl body, improving ultrastructure of neuron cell.