[关键词]
[摘要]
目的：观察新鲜羊膜移植对碱烧伤大鼠模型角膜缘干细胞增殖细胞核抗原(proliferating cell nuclear antigen，PCNA)表达的影响。
方法：SD大鼠40只40眼制作眼碱烧伤模型； 随机选取20只20眼行新鲜羊膜移植为实验组，对照组为不处理的烧伤模型眼。于术后1，2，3，4wk取角膜缘组织，采用免疫组化技术观察PCNA在角膜缘干细胞的表达情况。
结果：PCNA在实验组和对照组的角膜缘干细胞中均有表达，位于角膜缘上皮细胞基底层的细胞核内，但羊膜移植组显著高于对照组，两者比较有统计学意义(P<0.05)； 羊膜移植术后1，2，3，4wk时PCNA蛋白在角膜缘干细胞的表达不一致，呈线性趋势，1wk达到高峰，以后逐渐降低。
结论：眼碱烧伤后行羊膜移植术可促进角膜缘干细胞的增殖表达，利于角膜上皮的修复。
[Key word]
[Abstract]
AIM: To observe the expression of proliferating cell nuclear antigen(PCNA)in SD rats corneal limbus following alkali burns by amniotic membrane transplantation, and to explore the effect of limbal stem cells in alkali burned rats.
METHODS: Forty healthy SD rats forty eyes were randomly divided into experimental and control groups, with 20 animals in each group. After the preparation of corneal alkali burn models, cornea of rats in the experimental groups was given amniotic membrane transplantation, and control groups received no treatment. Five rats of each group were killed at weeks 1, 2, 3, 4 after operation. The expression of PCNA in limbal stem cells was detected with immunohistochemistry and computer image analysis system at different time points.
RESULTS: The expression of PCNA in experimental groups was higher than the control groups and there was significant difference(P<0.05). The expression of PCNA gradually decreased as time of alkali burn passed by.
CONCLUSION: Amniotic membrane transplantation can enhanced limbal stem cells proliferation in rats with corneal alkali burn and accelerate the healing of corneal epithelium.
[中图分类号]
[基金项目]
四川省卫生厅科研课题资助项目(No.060116)
