方法：通过免疫荧光及共聚焦显微镜的方法检验Cav-1在野生鼠视网膜中的表达； 通过HE染色的方法检测野生型(wild type，WT)和Cav-1敲除小鼠视网膜的形态及结构； 应用视网膜电流图的方法检测WT和Cav-1敲除小鼠视网膜的功能。

结果： Cav-1在视网膜的多种细胞中都有表达，在Müller细胞、视网膜血管和RPE中的表达尤为强烈。视网膜电流图表明，Cav-1敲除小鼠a波与b波均较WT鼠低，但HE染色显示其视网膜形态和结构没有明显异常。

结论：Cav-1缺陷小鼠表现出光感反应降低，但视网膜形态和结构均正常，提示Cav-1缺陷鼠视力下降并不源于光感受器细胞的功能缺陷，而是由于视网膜下微环境改变所致。

METHODS: The expression of Cav-1 was examined by immunofluorescence combined with confocal microscopy. Retinal structure in wild type(WT)and Cav-1 null mice was examined by HE staining. In vivo retinal function in WT and Cav-1 null mice was assessed by full-field scotopic electroretinography(ERG)following overnight dark adaptation.

RESULTS: Cav-1 null mice displayed reduced a-wave and b-wave amplitudes and reduced sensitivity as measured by ERG. However, the general structure of Cav^-/- retina was largely normal. Collectively, this implies the impairment of retinal function in Cav^-/- retina was not intrinsic to photoreceptor.

CONCLUSION: The observation that Cav-1 null mice show abnormal retinal function, in vivo but normal photoreceptor structure suggests that the retinal microenvironment rather than the photoreceptor itself is impaired. These may result in a disturbance in retinal pH, water or ion homeostasis therefore altering the subretinal milieu.