目的：研究体外环境下高糖培养的人视网膜血管内皮细胞(human retinal capillary endothelial cells， HRCEC)经过不同浓度的阿魏酸钠(sodium ferulate，SF)作用后的增殖以及血管内皮细胞生长因子(vascular endothelial growth factor， VEGF)表达的影响。

方法：对眼科手术新鲜的人角膜移植术后眼球进行取材并进行HRCEC的原代培养。进行实验的为生长良好的第3～4代细胞，实验分为空白对照组、低糖对照组、高糖对照组、高糖+不同浓度阿魏酸(1mg/L，2mg/L，3mg/L，4mg/L)组， 用噻唑蓝比色法(MTT)检测48h后不同浓度的阿魏酸钠对其增殖的抑制作用。免疫细胞化学法用于检测低糖对照组、高糖对照组和不同浓度阿魏酸(1mg/L，2mg/L，4mg/L)组HRCEC中VEGF的表达。

结果：MTT比色法结果表明： 48h后不同浓度的阿魏酸钠分别作用于原代培养的HRCEC细胞增生抑制率分别为46.97%，61.55%，76.91%和83.47%。一定质量浓度范围内阿魏酸钠对高糖环境下HRCEC具有明显的增殖抑制作用(P<0.05)。低糖对照组与高糖对照组吸光度A结果相比，差异无统计学意义(P=0.068>0.05)。低糖对照组、高糖对照组与浓度分别为1mg/L，2mg/L，4mg/L的阿魏酸钠作用组作用于HRCEC 48h后免疫细胞化学背景灰度值与阳性灰度值之差分别为28.27±1.62，93.67±0.81，72.67±2.89，53.73±1.70，30.93±3.72。通过48h后加药组和高糖对照组相比， VEGF表达下降明显，且均有统计学意义(均为P<0.05)，具有质量浓度依赖性。与低糖对照组相比，高糖对照组VEGF的表达明显增加(P<0.05)。

结论：阿魏酸钠对体外培养的高糖环境下HRCEC的增殖及高糖引起VEGF表达具有抑制作用。

AIM:To study the effects of different concentrations of sodium ferulate(SF)on human retinal capillary endothelial cells(HRCEC)proliferation and vascular endothelial growth factor(VEGF)expressing with different concentrations in vitro conditions of high glucose.

METHODS: The extracted from corneal transplantation HRSEC and cultured in vitro, which being good growth and at the 3- 4^th generation were taken into experiment. The experimental were divided into blank control group, high glucose control, low glucose control group, high glucose control group,high glucose+different concentrations of SF(1mg/L,2mg/L,4mg/L)group. The result of SF with different concentrations on the proliferation of HRCEC for 48 hours was surveyed by MTT assay. The immunocytochemical method was used to detect low glucose control group, high glucose group and different concentrations of SF(1mg/L, 2mg/L, 4mg/L)group HRCEC of VEGF expression.

RESULTS: The results of MTT colorimetric method showed that: different concentrations of SF in primary cultured HRCEC for 48 hours, cell proliferation inhibition rates were 46.97%, 61.55%, 76.91% and 83.47%. Within a certain range of concentrations of SF can restrain the proliferation of HRCEC in high glucose environment(P<0.05), and in 48 hours was concentration dependent. Low glucose control group compared with the high glucose control group the differences were not statistically significant(P=0.067>0.05). Low glucose control group, high glucose group and SF in concentrations of 1mg/L, 2mg/L, 4mg/L in HRCEC for 48 hours, immunocytochemistry of background gray values with positive gray value difference were respectively 28.27±1.62, 93.67±0.81, 72.67±2.89, 53.73±1.70, 30.93±3.72. Compared to the high glucose control group, expression of VEGF was decreased significantly in experimental groups after 48 hours, the difference was statically significant(P<0.05), in a dose-dependent manner. Compared to the low sugar glucose, VEGF in high glucose control group expression was significantly increased(P<0.05).

CONCLUSION: SF can reduce the HRCEC proliferation and suppress the expression of VEGF in high glucose environment which were cultured in vitro.