方法：以高浓度氧诱导C57BL/6J小鼠建立视网膜新生血管模型。取对照组和给氧组幼鼠眼球作荧光素血管灌注、病理切片及免疫组织化学检测，分别观察视网膜血管的改变、视网膜新生血管内皮细胞数目及Survivin和COX-2蛋白的表达。

结果：给氧组视网膜可见大量新生血管形成，新生血管内皮细胞核数为23.38±1.07个，与对照组相比差异有显著统计学意义(P<0.01)。正常对照组：视网膜组织各层中未见Survivin和COX-2蛋白表达(2.56±0.46，2.37±0.80)。给氧组：Survivin蛋白表达在神经节细胞层和突破视网膜内界膜的新生血管(3.34±0.40)，COX-2蛋白表达在内核层、神经节细胞层和突破视网膜内界膜的新生血管(3.89±0.56)。两者表达呈显著正相关(r=0.394，P<0.01)。

结论：在视网膜新生血管形成中存在Survivin和COX-2的高表达，且两者表达密切相关。

METHODS:Mouse model of hyperoxia-induced ischemic retinopathy were established. A novel flucorescein-dextran perfusion method was developed to assess the vascular pattern. Histologic methods were used to count blood vessel profiles in the inner retina with HE staining. Survivin and COX-2 expressions in the retina were determined by immunohistochemical method.

RESULTS: Lots of neovascularization were seen in hyperoxia group. The number of nuclei of proliferative retinal vessels increased significantly as compared with normal control group(P<0.01). The expression of Survivin was noted in the ganglion cells and the neovascularization breaking through the inner retinal. The expression of COX-2 was noted in the inner nuclear layer and ganglion cells and retinal blood vessels. The expressions of them have positive correlation(r=0.394,P<0.01).

CONCLUSION:There are high expressions of Survivin and COX-2 protein in the retinal neovascularization and they have correlation.