方法：分别将4组PAX6 shRNA慢病毒载体及对照组慢病毒(pGCL-GFP-shRP1，2，3，4，NC)感染B3细胞； 感染96h后，利用Real-time PCR和Western-blot检测B3细胞PAX6表达以确定PAX6沉默； 选取有效沉默PAX6基因的pGCL-GFP-shRP4感染B3细胞，MOI=10，观察细胞数量变化。感染48h后采用流式细胞仪检测B3细胞凋亡情况。

结果：感染RNAi病毒组的B3细胞，随感染时间延长，细胞数量减少。流式细胞仪检测结果显示沉默PAX6 48h的B3细胞，G₁/G₀期前出现凋亡峰。

结论：慢病毒介导的RNA干扰能有效沉默B3细胞的PAX6表达； PAX6基因沉默后B3细胞凋亡。

METHODS: Four groups PAX6 shRNA lentiviral vectors and the control group lentiviral(pGCL-GFP-shRP1, 2, 3, 4, NC)infect B3 cells. Real Time PCR and Western blot analysis was used to determine PAX6 silence after infection 96 hours. Effective PAX6-silence pGCL-GFP-shRP4 was selected to infect B3 cells, MOI(Multiplicity of infection)= 10 and the changes were observed in the number of the cells. The B3 cells apoptosis was detect by flow cytometry after 48 hours in infection.

RESULTS: RNAi-virus-infected B3 cells decreased in the number of cells with infection prolonged. The flow cytometry results showed that after knock-down the PAX6 for 48 hours, apoptotic peak appeared before the G₁/G₀ phase.

CONCLUSION: Lentivirus-mediated RNA interference can effectively silence PAX6 expression in B3 cells. There is HLE-B3 apoptosis after PAX6 gene knock-down.