方法：RF/6A细胞分4组培养(n=6/组)，对照组：DMEM培养基+100/L胎牛血清； 高糖组：对照组培养基添加40mmol/L葡萄糖； 姜黄素组：对照组培养基添加30μmol/L姜黄素； 姜黄素-高糖组：对照组培养基添加40mmol/L葡萄糖及30μmol/L姜黄素。分组培养5d后，显微镜观察各组细胞生长状态，噻唑蓝比色法(methyl thiazolyl tetrazolium，MTT)检测各组细胞活力，琼脂糖凝胶电泳检测DNA ladder判断凋亡，Western-blot检测肿瘤坏死因子-α(tumor necrosis factor-α，TNF-α)的表达。

结果：与对照组相比，高糖组RF/6A细胞生长状态较差，活力明显降低(P<0.01)，而姜黄素组及姜黄素-高糖组RF/6A活力无明显变化(P>0.05)。高糖组RF/6A出现明显的DNA ladder，对照组、姜黄素组以及姜黄素-高糖组未见明显DNA ladder。与对照组相比，高糖组TNF-α表达上调(P<0.01)，姜黄素组及姜黄素-高糖组TNF-α表达无明显变化(P>0.05)。

结论：姜黄素抑制高浓度葡萄糖诱导的RF/6A细胞凋亡、维持细胞活力，可能与姜黄素恢复高浓度葡萄糖诱导的TNF-α表达有关。

METHODS: RF/6A was randomly assigned into 4 groups(n=6/group): Control group(CONT group, DMEM+10% FBS), high glucose concentration group(HGC group, DMEM+10% FBS+40mmol/L glucose), curcumin group(CUR group, DMEM+10% FBS+30μmol/L curcumin)and curcumin-glucose group(C-G group, DMEM+10% FBS+40mmol/L glucose+30μmol/L curcumin). Five days later, cells were observed with microscope, and cell viability was detected by methyl thiazolyl tetrazolium(MTT). Moreover, agarose gel electrophoresis was introduced to investigate DNA ladder in each group. We also detected expression of tumor necrosis factor-α(TNF-α).

RESULTS: Compared with CONT group, cell viability in HGC group was decreased(P<0.01), showing no obvious difference in CUR or C-G groups(P>0.05). HGC group presented obvious DNA ladder, while no ladder was observed in CONT, CUR or C-G group. Furthermore, compared with CONT group, TNF-α was up-regulated in HGC group(P<0.01), and no difference was detected in CUR group or C-G group(P>0.05).

CONCLUSION: Curcumin attenuates high glucose concentration induced RF/6A apoptosis, maintaining cell viability, which may result from reversing HGC-induced up-regulation of TNF-α.