方法：对78例156眼在我院实施去上皮瓣Epi-LASIK手术后的近视患者随机分为两组，A组为小牛血去蛋白提取物组， 40例80眼； B组为对照组，38例76眼。两组术后常规给予妥布霉素地塞米松滴眼液、羟糖甘滴眼液4次/d，共7d，之后常规滴1g/L氟米龙滴眼液，4次/d，逐月递减1次，共用4mo。A组在此基础上，加用小牛血蛋白提取物眼用凝胶，4次/d，共7d。 术后随访6mo，定期观察两组术后反应、角膜上皮愈合时间、视力及角膜上皮下雾状混浊(haze)情况。

结果：术后A组角膜上皮平均愈合时间为2.89±1.06d，对照组为4.22±1.47d，小牛血组明显短于对照组，上皮愈合更快(t=6.45，P<0.05)； 术后第1d和第2d，A组反应程度评分为1.70±0.44和1.02±0.60，B组为2.89±0.43和1.71±0.55，小牛血组术后反应程度明显轻于对照组(P<0.05)。3d后术后反应两组差异均无统计学意义(P>0.05)； 两组患眼术后视力恢复和haze情况差异均无统计学意义(P>0.05)。

结论：去上皮瓣Epi-LASIK术后应用小牛血去蛋白提取物眼用凝胶能够减轻术后反应，加快角膜上皮愈合，起效时间短，安全性较好。

METHODS: Seventy-eight patients(156 eyes)performing off-flap Epi-LASIK were randomized into experimental and control groups. Forty patients(80 eyes)were in experimental group and thirty-eight patients(76 eyes)in control group. The two groups were treated with compound tobramycin eye drops, tears naturale eye drops for 7 days, four times a day, then the conventional artificial tears and 1g/L fluorometholone were given. Protein-free calf blood extract eye gel for 7 days, four times a day were given in the experimental group, the follow-up time was 6 months, the postoperative pain, time of corneal epithelial recovery, visual acuity and corneal subepithelial opacity(haze)were examined at special time in two groups. Clinical outcomes were compared between groups.

RESULTS: The mean time of corneal epithelial recovery was(2.89±1.06)days in the experimental group and(4.22±1.47)days in the control group. Compared with the control group, outcome of rapider reepithelialization was found in the experimental group, there was statistical difference(t=6.45, P<0.05). The average pain score of experimental group was(1.70±0.44)on the first day and(1.02±0.60)on the second day, the control group was(2.89±0.43)and(1.71±0.55), the experimental group obviously had milder pain, there was statistical difference(P<0.05). There was no significant difference in visual acuity and haze between the two groups(P >0.05).

CONCLUSION: Protein-free calf blood extract eye gel has effectiveness on reducing ocular pain and promoting cornea epithelial healing after Epi-LASIK, the application is safe and has shorter onset time.