方法：采用离体晶状体培养技术，在一定浓度H₂O₂的MEM培养液中置入透明的SD大鼠晶状体，建立实验性白内障晶状体模型。设置空白对照组、H₂O₂组和茶多酚(0.02g/L，0.2g/L，2g/L)处理组，分别于不同时间点(6，12，24，48h)取样，应用RT-PCR检测晶状体上皮细胞中NF-κB mRNA表达，Western-blot检测晶状体上皮细胞中NF-κB蛋白表达。

结果：H₂O₂上调晶状体上皮细胞中NF-κB的表达，且呈时间依赖性(P<0.01)； 不同浓度的茶多酚均可抑制晶状体上皮细胞中NF-κB的表达，茶多酚浓度为0.2g/L时抑制效果最显著(P<0.05)。

结论：茶多酚可抑制H₂O₂诱导的晶状体上皮细胞中NF-κB的表达，并且在茶多酚浓度为0.2g/L时抑制效果最明显。

METHODS: SD rats transparent crystals were cultured in vitro by lens technology, and placed in a certain concentration of H₂O₂ in MEM medium. The experimental cataract lens model was established. Blank control group, H₂O₂ group and tea polyphenols(0.02mg/mL, 0.2mg/mL, 2mg/mL)treatment group were set and sampled at different time points(6, 12, 24, 48 hours). NF-κB mRNA expression was detected by RT-PCR, and NF-κB protein expression was detected by Western-blot in lens epithelial cells.

RESULTS: H₂O₂ raised the expression of NF-κB, and appeared time-dependent manner(P<0.01)in lens epithelial cells; different concentrations of tea polyphenols can inhibit the expression of NF-κB in lens epithelial cells, when TP concentration was 0.2mg/mL, the inhibitory effects was statistically significant(P<0.05).

CONCLUSION: TP can inhibit the expression of NF-κB in H₂O₂-induced lens epithelial cells, when TP concentration was 0.2mg/mL, the effect is statistically significant.