目的：观察脑苷肌肽(CEGI)对视网膜变性模型(rds小鼠)发育过程的影响，旨在探求CEGI治疗视网膜变性类疾病的治疗效果，为临床用药提供客观依据和指导。

方法：采用组织病理学技术(光、电镜)和原位末端转移酶标记(TUNEL)方法观察脑苷肌肽腹腔给药干预后视网膜组织形态、超微结构、感光细胞凋亡的变化。

结果：生后14d(P14)是rds鼠视网膜发育最完善阶段，随后28～56d，视网膜外核层及内核层细胞层数逐渐减少，感光细胞凋亡细胞数逐渐增多，电镜观察有凋亡核变化以及内节和纤毛崩解。给予CEGI治疗后28d和56d，视网膜细胞层数较同日龄对照组增厚，凋亡细胞数减少，有统计学差异(P<0.05)。神经生长因子(NGF)与CEGI作用相似。

结论：脑苷肌肽有促进视网膜细胞生长发育作用，对rds小鼠视网膜变性过程有一定的缓解作用。

AIM: To explore the protective effects of cattle encephalon glycoside and ignotin(CEGI)on developmental process of rds mice, investigate its function and effects on retinal degenerative diseases, and offer the basis on treating similar diseases.

METHODS:The retinal pathologic and ultrastructure changes of rds mice treated by CEGI were observed by light and electron microscopy, and the apoptosis rates of photoreceptor cells were determined through TUNEL(tdt- mediated biotin-dUTP nick-end labeling)technique.

RESULTS: Postnatal 14 days(P14)was the best stage of developmental process of rds mice. After 28-56 days, cell number on retinal outer nuclear layer and retinal inner layer reduced, and photoreceptor apoptosis increased gradually. What's more, electron microscopy showed apoptosis nuclear changes and disintegration of inner section and cilia. On 28 days and 56 days, CEGI groups showed thicker retinal cell layers and less apoptotic cells compared with the control group. There was no statistically significant difference in comparison. Nerve growth factor(NGF)had similarity function with CEGI.

CONCLUSION: CEGI can promote growth and development of retinal cells, and have a protective effect on retinal degenerative process of rds mice.