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[摘要]
目的:探讨浸润性树突状细胞(TIDC)在视网膜母细胞瘤(retinoblastoma,Rb)组织中的表达。
方法:以鼠抗人CD1a单克隆抗体为一抗,采用免疫组化SP染色法检测31例Rb组织中树突状细胞的表达。
结果:在所有31例标本中,有23例经免疫组化染色后有CD1a+细胞的表达,即阳性率为74.2%。其中高分化为主型阳性率为77.8%(7/9),未分化为主型阳性率为75%(15/20),自发退变型为50%(1/2)。
结论: Rb组织中CD1a阳性细胞数量与肿瘤分型之间未见明显相关性,TIDC是否可看作一个良好的肿瘤预后指标,还有待进一步确证。
[Key word]
[Abstract]
AIM: To study the expression of tumor-infiltrating dendritic cells(TIDC)in retinoblastoma(Rb)and its prognostic significance.
METHODS: The expression of tumor-infiltrating dendritic cells was examined by immunohistochemical staining(S-P method), using CD1a mouse monoclonal antibody in Rb tissues of 31 cases.
RESULTS:The expression of CD1a+ cells was found in tumor samples of 23 cases(74.2%)among the total 31 cases. The rates of positive CD1a expression in well-differentiated type was 77.8%(7/9)and in undifferentiated type cancer tissues was 75%(15/20), respectively(P>0.05). The rate of spontaneous regression type was 50%(1/2).
CONCLUSION: There was no correlation between the number of CD1a+ cells and tumor grade in Rb. It is difficult to identify whether TIDC was good indicator in assessing tumor prognosis.
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