方法：SS性干眼的8周龄雌性NOD模型小鼠20只，随机分为4组，每组5只，9周龄时开始给药。另有5只8周龄健康KM小鼠作为正常对照组。实验Ⅰ组和Ⅱ组小鼠分别给予200μg/kg和400μg/kg的雷帕霉素缓释微球结膜下注射，实验Ⅲ组和Ⅳ组动物分别给予生理盐水和空白微球载体结膜下注射。用药前及用药后第5，10，15，20d检查各组小鼠的泪液分泌量，对角膜行荧光素染色并评分，对角结膜行虎红染色并评分，用结膜印记细胞学方法检测结膜细胞并分级。

结果：实验组与对照组相比，泪液分泌量明显增加，角膜荧光素染色和角结膜虎红染色评分降低，结膜印记细胞学等级降低。

结论：雷帕霉素缓释微球可以通过抑制细胞免疫反应，增加NOD小鼠泪液分泌量，对NOD小鼠干燥综合征性干眼具有显著疗效。

METHODS: Twenty 8-week-old female NOD mice with dry eye caused by SS were randomly divided into 4 groups. One week later, the mice were treated with subconjunctival injection. GroupⅠ and Ⅱ received 200μg/kg and 400μg/kg rapamycin-loaded microspheres, Group Ⅲ and Ⅳ received normal saline and empty microspheres. Five 8-week-old female healthy KM mice were used as untreated controls. Before and 5, 10, 15, 20 days after the experiment, the amount of secretion of tears, the score of corneal fluorescein staining and rose bengal staining were investigated. Conjunctival epithelial cells were observed and graded by conjunctival impression cytology.

RESULTS: Compared with the group Ⅲ and Ⅳ, the amount of secretion of tears of the mice in groupⅠ and Ⅱ increased. The scores of corneal fluorescein staining and rose bengal staining were lower. The levels of conjunctival impression cytology reduced.

CONCLUSION: Rapamycin-loaded microspheres can decrease dry eye signs by alleviating the ocular surface inflammation of NOD mice. It suggests rapamycin-loaded microsphere is valuable to dry eye caused by SS.