方法：人晶状体上皮细胞系传代培养，紫外线诱导细胞发生凋亡， 20μmol/L白藜芦醇预处理细胞后，观察各项指标改变：流式细胞仪检测细胞凋亡率，比色法检测凋亡相关因子caspase-3及caspase-9的表达，透射电镜观察超微结构变化。

结果：流式细胞仪检测发现，白藜芦醇可以抑制紫外线照射诱导的细胞凋亡，阳性对照组中caspase-3及caspase-9含量显著高于同时段阴性对照组，差异有统计学意义(P<0.05)； 实验组中caspase-3及caspase-9含量均低于同时段阳性对照组，差异有统计学意义(P<0.05)。此外，伴随白藜芦醇作用时间的延长， 透射电镜下人晶状体上皮细胞损伤的程度减轻。

结论：白藜芦醇可以抑制紫外线诱导的晶状体上皮细胞的凋亡，对辐射性白内障具有预防作用，从而为寻求有效的防治白内障药物提供可靠的实验依据。

METHODS:Subcultured human lens epithelial cell line, ultraviolet induced cell apoptosis, 20μmol/L resveratrol pretreated cell, the indicators change was observed: rate of apoptosis was detected by flow cytometry and apoptosis-related factors of caspses-3 and caspase-9 were detected by colorimetric detection, ultrastructure changes were observed under transmission electron microscope.

RESULTS: Flow cytometry instrument testing found that resveratrol can suppress the apoptosis induced by ultraviolet irradiation, caspses-3 and caspase-9 content in positive control group were significantly higher than that of the negative control group at the same time period, the difference was statistically significant(P<0.05); caspses-3 and caspase-9 content in experimental group were lower than that in the positive control group at the same time, the difference was statistically significant(P<0.05). In addition, the damage of human lens epithelial cells was alleviated with the incubation time of resveratrol elongated.

CONCLUSION:Resveratrol may inhibit ultraviolet-induced apoptosis of human lens epithelial cells, it has preventive function against radioactive cataract, and it can provide reliable evidence for pursuing effective medicine to prevent and treat cataract.