方法：选择我院2001-01/2012-12收存在眼科病理室的15例Rb组织切片设为观察组，再选取15例正常视网膜组织切片设为对照组。应用Western-Blot及RT-PCR(逆转录酶链反应)法分别对正常视网膜组织及Rb组织中的Pax6蛋白和Pax6 mRNA的表达进行检测，同时应用Western-Blot法对Pax6基因下游的BRN3b及MATH5促分化基因在蛋白水平的表达进行检测，最后进行组间比较，进而对Pax6基因在Rb中的表达及临床意义进行探讨。

结果：观察组Pax6基因mRNA表达平均值为0.99±0.03，Pax6基因蛋白表达平均值为2.07±0.15，BRN3b蛋白表达平均值为0.195±0.016，MATH5蛋白表达平均值为0.190±0.031，均明显高于对照组，差异有统计学意义(P<0.05)。

结论：异常表达的Pax6基因可能对Rb的出现起到促进作用。

METHODS: Totally 15 cases of fresh Rb organizations were selected as observation group and 15 normal retinal organizations as control group. Western-Blot and reverse transcriptase polymerase chain reaction(RT-PCR)methods were used to detect Pax6 protein and Pax6 mRNA expressions of the normal retina organizations and Rb organizations. At the same time, Western Blot method was used to detect the Pax6 gene downstream MATH5 and BRN3b differentiation gene protein level expression. After the comparison between two groups, the expression and clinical significance of Pax6 gene in Rb were discussed.

RESULTS: In the observation group, average value of mRNA expression of Pax6 gene was 0.99±0.03; average value of Pax6 gene protein expression was 2.07±0.15; average value of BRN3b protein expression was 0.195±0.016; average value of MATH5 protein expression was 0.190±0.031. They were significantly higher than the control group, and the differences were statistically significant(P<0.05).

CONCLUSION: Abnormal expression of Pax6 gene is likely to accelerate the occurrence of Rb.